About: McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts

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About: McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Despite advances in antifungal prophylaxis and therapy, morbidity and mortality incurred by yeasts remain a significant burden. As pathogenic yeast species vary in their susceptibilities to antifungal agents, clinical microbiology laboratories face an important challenge to identify them rapidly and accurately. Although a vast array of phenotyping and genotyping methods has been developmed these are either unable to cover the whole spectrum of potential yeast pathogens or can do this only in a rather costly or laborious way. Random amplified polymorphic DNA (RAPD) fingerprinting was repeatedly demonstrated to be a convenient tool for species identification in pathogenic yeasts. However, its wider acceptance has been limited mainly due to special expertise and software needed for analysis and comparison of the resulting banding patterns. Based on a pilot study, we demonstrate here that a simple and rapid melting curve analysis of RAPD products can provide data for identification of five of the most Despite advances in antifungal prophylaxis and therapy, morbidity and mortality incurred by yeasts remain a significant burden. As pathogenic yeast species vary in their susceptibilities to antifungal agents, clinical microbiology laboratories face an important challenge to identify them rapidly and accurately. Although a vast array of phenotyping and genotyping methods has been developmed these are either unable to cover the whole spectrum of potential yeast pathogens or can do this only in a rather costly or laborious way. Random amplified polymorphic DNA (RAPD) fingerprinting was repeatedly demonstrated to be a convenient tool for species identification in pathogenic yeasts. However, its wider acceptance has been limited mainly due to special expertise and software needed for analysis and comparison of the resulting banding patterns. Based on a pilot study, we demonstrate here that a simple and rapid melting curve analysis of RAPD products can provide data for identification of five of the most (en) Náhodná aplifikace polymorfní DNA (RAPD) se opakovaně ukázala jako vhodný nástroj pro druhovou identifikaci patogenních kvasinek. Na základě pilotní studie demonstrujeme, že jednoduchá a rychlá metoda analýzy teplotní křivky RAPD produktů umožňuje identifikaci pěti lékařsky nejvýznamnějších druhů kandid. (cs)
Title	McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts (en) McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts (cs)
skos:prefLabel	McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts (en) McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts (cs)
skos:notation	RIV/61989592:15110/05:00002046!RIV06-MZ0-15110___
http://linked.open.../vavai/riv/strany	107-113
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(NI7498)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Hamal, Petr Raclavský, Vladislav
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	529149
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/05:00002046
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Candida; identification; RAPD; Melting curve; analysis (en)
http://linked.open.../riv/klicoveSlovo	Candida analysis identification RAPD Melting curve
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[CD31DAEFD1A0]
http://linked.open...i/riv/nazevZdroje	Journal of Microbiological Methods
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Rapid PCR detection and epidemiology of nosocomial invasive aspergillosis
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	60
http://linked.open...iv/tvurceVysledku	Hamal, Petr Raclavský, Vladislav Plachý, Radek
issn	0167-7012
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	15110

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