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| Description
| - Byl sledován vliv neesterifikovaných mastných kyselin (NEFA) a hormonu dehydroepiandrosteronu na hladiny mRNA isoforem δ a  proteinkinázy C (PKC) a mRNA liver fatty acid binding proteinu (L-FABP) v lidských hepatomových buňkách HepG2. Buňky byly po dobu experimentu udržovány v mediu s nízkým obsahem séra a albuminu. Zvolená koncentrace mastných kyselin byla 100 µM a časové intervaly 4 a 20 hodin. Za těchto podmínek nasycené (palmitová, stearová) a mononenasycené (olejová) kyseliny poměrně selektivně stimulovaly mRNA PKC . Neočekávaně jsme zjistili, že tyto mastné kyseliny také snižují hladinu mRNA L-FABP. DHEA ve farmakologických dávkách (100 μM) vyvolává znatelné zvýšení hladin mRNA PKC-δ a -ε. I když v tomto příspěvku nebyly zkoumány molekulární mechanismy na pozadí zjištěných změn, naše výsledky zdůrazňují, že modulace hladin mRNA, indukované NEFA, představují další mechanismus, kterým mohou NEFA v prostředí ovlivňovat metabolickou homeo (cs)
- The effects of non-esterified fatty acids (NEFA) and hormone dehydroepiandrosterone (DHEA) on the levels of mRNAs of protein kinase C (PKC) -δ and -ε isoforms and those of liver fatty acid binding protein (L-FABP) were investigated in the human hepatoma HepG2 cell line. The cells were kept in low-serum, low-albumin medium during experiments. Low FA levels (100 μM) and time intervals of 4 h and 20 h were used. In these conditions, the saturated (palmitic, stearic) and monounsaturated (oleic) acids rather selectively stimulated PKC-ε mRNA levels. Unexpectedly, we found that these acids also suppressed liver fatty-acid binding protein (L-FABP) mRNA levels. DHEA in pharmacological doses (100 μM) produced a significant increase in PKC-δ and -ε mRNA levels. Although molecular mechanisms underlying the identified changes have not been investigated in this paper, our findings emphasize that NEFA-induced modulation of mRNA levels of key signalling
- The effects of non-esterified fatty acids (NEFA) and hormone dehydroepiandrosterone (DHEA) on the levels of mRNAs of protein kinase C (PKC) -δ and -ε isoforms and those of liver fatty acid binding protein (L-FABP) were investigated in the human hepatoma HepG2 cell line. The cells were kept in low-serum, low-albumin medium during experiments. Low FA levels (100 μM) and time intervals of 4 h and 20 h were used. In these conditions, the saturated (palmitic, stearic) and monounsaturated (oleic) acids rather selectively stimulated PKC-ε mRNA levels. Unexpectedly, we found that these acids also suppressed liver fatty-acid binding protein (L-FABP) mRNA levels. DHEA in pharmacological doses (100 μM) produced a significant increase in PKC-δ and -ε mRNA levels. Although molecular mechanisms underlying the identified changes have not been investigated in this paper, our findings emphasize that NEFA-induced modulation of mRNA levels of key signalling (en)
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| Title
| - Changes in mRNA levels of intracellular fatty acid metabolism regulators in human hepatoma HepG2 cells following their treatment with non-esterified fatty acids and dehydroepiandrosterone
- Changes in mRNA levels of intracellular fatty acid metabolism regulators in human hepatoma HepG2 cells following their treatment with non-esterified fatty acids and dehydroepiandrosterone (en)
- Změny v hladinách mRNA intracelulárních regulátorů mastných kyselin působením neesterifikovaných mastných kyselin a dehydroepiandrosteronu na lidské hepatomové buňky HepG2 (cs)
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| skos:prefLabel
| - Changes in mRNA levels of intracellular fatty acid metabolism regulators in human hepatoma HepG2 cells following their treatment with non-esterified fatty acids and dehydroepiandrosterone
- Changes in mRNA levels of intracellular fatty acid metabolism regulators in human hepatoma HepG2 cells following their treatment with non-esterified fatty acids and dehydroepiandrosterone (en)
- Změny v hladinách mRNA intracelulárních regulátorů mastných kyselin působením neesterifikovaných mastných kyselin a dehydroepiandrosteronu na lidské hepatomové buňky HepG2 (cs)
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| skos:notation
| - RIV/61989592:15110/05:00001984!RIV07-MSM-15110___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(1P05OC063), P(1P05OC064), P(OC B17.20), Z(MSM6198959205)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/05:00001984
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Fatty acids; Protein kinase C (PKC); Liver fatty acid binding protein (L-FABP); Insulin resistance; HepG2 cells (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Veselý, Jaroslav
- Uherková, Lenka
- Rypka, Miroslav
- Bogdanová, Kateřina
- Poczatková, Hana
- Červenková, Kateřina
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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