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| Description
| - Four beta-glycosides of flavonoligan silybin, i.e. silybin beta-galactoside, silybin beta-glucoside, silybin beta-maltoside, silybin beta-lactoside were synthesized in order to improve silybin water solubility and bioavailability (Kren et al., J Chem Soc, Perkin Trans 1, 2467-2474, 1997). The presented paper deals with the effect of silybin and its synthetic beta-glycosides on the expression of two major cytochrome P450 isoforms, CYP1A2 and CYP3A4. Primary cultures of human hepatocytes were the model of choice. mRNAs were analyzed using Northern blot and P-radiolabelled probes. CYP protein content was determined by immunoblotting using specific antibodies. Silybin and its beta-glycosides do not induce expression of CYP1A2 and CYP3A4. Tested compounds did not affect inducible expression of CYP1A2 and CYP3A4 by dioxin and rifampicin, respectively, as evaluated at the level of mRNAs and proteins. Silybin and its beta-glycosides do not interfere with the expression of CYP1A2 and CYP3A4, are not likely to
- Four beta-glycosides of flavonoligan silybin, i.e. silybin beta-galactoside, silybin beta-glucoside, silybin beta-maltoside, silybin beta-lactoside were synthesized in order to improve silybin water solubility and bioavailability (Kren et al., J Chem Soc, Perkin Trans 1, 2467-2474, 1997). The presented paper deals with the effect of silybin and its synthetic beta-glycosides on the expression of two major cytochrome P450 isoforms, CYP1A2 and CYP3A4. Primary cultures of human hepatocytes were the model of choice. mRNAs were analyzed using Northern blot and P-radiolabelled probes. CYP protein content was determined by immunoblotting using specific antibodies. Silybin and its beta-glycosides do not induce expression of CYP1A2 and CYP3A4. Tested compounds did not affect inducible expression of CYP1A2 and CYP3A4 by dioxin and rifampicin, respectively, as evaluated at the level of mRNAs and proteins. Silybin and its beta-glycosides do not interfere with the expression of CYP1A2 and CYP3A4, are not likely to (en)
- Čtyři ß-glykosidy flavonolignanu silybinu, silybin ß -galaktosid, silybin ß-glukosid, silybin ß-maltosid a silybin ß-laktosid byly syntetizovány za účelem zvýšení rozpustnosti silybinu ve vodě a jeho biodostupnosti (Křen et al., J Chem Soc, Perkin Trans 1, 2467-2474, 1997). Článek se zabývá ovlivněním exprese CYP1A2 a CYP3A4, dvou hlavních cytochromů P450 silybinem a jeho syntetickými ß-glykosidy. Jako model byly použity primární kultury lidských hepatocytů, mRNA byla analyzována pomocí Northern blotu a 32P značené sondy. Proteiny cytochromů P450 byly stanovovány imunoblotem s použitím specifických protilátek. Silybin a jeho ß-glykosidy neindukují expresi CYP1A2 a CYP3A4.. Testované sloučeniny neovlivňují inducibilní expresi CYP1A2 a CYP3A4, indukovanou dioxinem, respektive rifampicinem, na úrovni mRNA a proteinů. Silybin a jeho ß-glykosidy neinterferují s expresí CYP1A2 a CYP3A4, proto by neměly vyvolávat mezilékové interakce, pramenících z indukce dvou důležitých cytochromů P450. (cs)
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| Title
| - Effect of silybin and its glycosides on the expression of cytochromes P450 1A2 and 3A4 in primary cultures of human hepatocytes
- Effect of silybin and its glycosides on the expression of cytochromes P450 1A2 and 3A4 in primary cultures of human hepatocytes (en)
- Vliv silybinu a jeho glykosidů na expresi cytochromů P450 1A1 a 3A4 v primárních kulturách lidských hepatocytů (cs)
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| skos:prefLabel
| - Effect of silybin and its glycosides on the expression of cytochromes P450 1A2 and 3A4 in primary cultures of human hepatocytes
- Effect of silybin and its glycosides on the expression of cytochromes P450 1A2 and 3A4 in primary cultures of human hepatocytes (en)
- Vliv silybinu a jeho glykosidů na expresi cytochromů P450 1A1 a 3A4 v primárních kulturách lidských hepatocytů (cs)
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| skos:notation
| - RIV/61989592:15110/05:00001963!RIV06-MSM-15110___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/05:00001963
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Human hepatocytes; silybin; silybin beta-glycosides; P450 (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Biochemical and Molecular Toxicology
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Dvořák, Zdeněk
- Kosina, Pavel
- Ulrichová, Jitka
- Maurel, Patrick
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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