About: Comparison of %22high throughput%22 micromethods for determination of cytochrome P450 activities with classical methods using HPLC for product identification

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About: Comparison of %22high throughput%22 micromethods for determination of cytochrome P450 activities with classical methods using HPLC for product identification Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Enzyme activities of the CYP enzymes (CYP3A4, CYP2C9 and CYP2A6) were determined using classical substrates (testosterone, diclofenac and coumarin, respectively) as well as with luminogenic or fluorogenic substrates in micromethod arrangement. The luciferin-based luminogenic substrates for CYP3A4 and CYP2C9 as well as coumarin in micromethod for assay of CYP2A6 activity gave results well comparable with the classical methods with determination of reaction products by the HPLC. Enzyme activities of the CYP enzymes (CYP3A4, CYP2C9 and CYP2A6) were determined using classical substrates (testosterone, diclofenac and coumarin, respectively) as well as with luminogenic or fluorogenic substrates in micromethod arrangement. The luciferin-based luminogenic substrates for CYP3A4 and CYP2C9 as well as coumarin in micromethod for assay of CYP2A6 activity gave results well comparable with the classical methods with determination of reaction products by the HPLC. (en) Metody studia aktivit cytochromů P450 s detekcí produktů pomocí HPLC lze úspěšně převést do mikroměřítka s využitím detekce na principu luminiscence nebo fluorescence. Tyto metody je možné použít i ke získání základních parametrů enzymové reakce. (cs)
Title	Comparison of %22high throughput%22 micromethods for determination of cytochrome P450 activities with classical methods using HPLC for product identification Srovnání %22high throughput%22 mikrometod stanovení aktivit cytochromu P450 s klasickými metodami používajícími pro identifikaci produktu HPLC (cs) Comparison of %22high throughput%22 micromethods for determination of cytochrome P450 activities with classical methods using HPLC for product identification (en)
skos:prefLabel	Comparison of %22high throughput%22 micromethods for determination of cytochrome P450 activities with classical methods using HPLC for product identification Srovnání %22high throughput%22 mikrometod stanovení aktivit cytochromu P450 s klasickými metodami používajícími pro identifikaci produktu HPLC (cs) Comparison of %22high throughput%22 micromethods for determination of cytochrome P450 activities with classical methods using HPLC for product identification (en)
skos:notation	RIV/61989592:15110/05:00001932!RIV06-MSM-15110___
http://linked.open.../vavai/riv/strany	353-356
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Mašek, Vlastimil ANZENBACHER, Pavel Anzenbacherová, Eva
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	515874
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/05:00001932
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cytochrome P450; CYP; luminiscence; fluorescence; HPLC (en)
http://linked.open.../riv/klicoveSlovo	luminiscence Cytochrome P450 fluorescence HPLC CYP
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[63CB2269845E]
http://linked.open...i/riv/nazevZdroje	Biomedical Papers
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	148
http://linked.open...iv/tvurceVysledku	Anzenbacher, Pavel Anzenbacherová, Eva Mašek, Vlastimil Veinlichová, Alena
http://linked.open...n/vavai/riv/zamer	Modulation of signalling and regulatory pathways in normal and cancer cells
issn	1213-8118
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	15110

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