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Description
  • This work presents method validation of fluoride determination in urine of children. The fluoride ion selective electrode and silver chloride reference electrode have been used for all measurements. We hawe found that described method is accurate (average recovery 98,4 %) and provided precision RSD=2,8 %- The evaluated value of limit of detection using direct signal method has been 3.2.10-6 mol.l-1. The fluoride concentration in real urine samples is influenced during the storage in the refrigerator (+ 4oC). The samples should be stored deeply frozen at -20 oC before the analysis. The comparison of several buffers (commercially available TISAB, citrate buffer with different pH and citrate buffer with NaCl) leads to the conclusion that there is no significant difference among used buffer solutions.
  • This work presents method validation of fluoride determination in urine of children. The fluoride ion selective electrode and silver chloride reference electrode have been used for all measurements. We hawe found that described method is accurate (average recovery 98,4 %) and provided precision RSD=2,8 %- The evaluated value of limit of detection using direct signal method has been 3.2.10-6 mol.l-1. The fluoride concentration in real urine samples is influenced during the storage in the refrigerator (+ 4oC). The samples should be stored deeply frozen at -20 oC before the analysis. The comparison of several buffers (commercially available TISAB, citrate buffer with different pH and citrate buffer with NaCl) leads to the conclusion that there is no significant difference among used buffer solutions. (en)
  • Tato práce seznamuje s validací metody určení fluoridu v moči u dětí. Pro všechna měření byly použity referenční elektrody citlivé na ionty fluoridu a chloridu stříbrného. Zjistili jsme, že popisovaná metoda je přesná (dosažený průměr 98,4 %) a daná přesnost RSD = 2,8 %. (cs)
Title
  • Stanovení fluoridu v moči pomocí iontově selektivní elektrody: v průběhu optimalizace metody a skladování vzorku (cs)
  • Fluoride determination in urine with ion selective electrode: within laboratory method and sample storage optimization
  • Fluoride determination in urine with ion selective electrode: within laboratory method and sample storage optimization (en)
skos:prefLabel
  • Stanovení fluoridu v moči pomocí iontově selektivní elektrody: v průběhu optimalizace metody a skladování vzorku (cs)
  • Fluoride determination in urine with ion selective electrode: within laboratory method and sample storage optimization
  • Fluoride determination in urine with ion selective electrode: within laboratory method and sample storage optimization (en)
skos:notation
  • RIV/61989592:15110/04:00007231!RIV09-MSM-15110___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM 153100013)
http://linked.open...iv/cisloPeriodika
  • 43
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 564624
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15110/04:00007231
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • fluoride; urine; method validation and sample storage (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [D35B2EF9E806]
http://linked.open...i/riv/nazevZdroje
  • Acta Universitatis Palackianae Olomucensis, Facultas Rerum Naturalium, Chemica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 43
http://linked.open...iv/tvurceVysledku
  • Milde, David
  • Nováková, Květoslava
http://linked.open...n/vavai/riv/zamer
issn
  • 0232-0061
number of pages
http://localhost/t...ganizacniJednotka
  • 15110
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