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| Description
| - The human glucocorticoid receptor (hGR) plays a pivotal role in cellular processes such as development, differentiation, homeostasis, immune response and in regulation of xenobiotic metabolism. It has been demonstrated recently that colchicine inhibits hGR transcriptional activity in primary cultures of human hepatocytes by a mechanism involving impairment of hGR nucleo-cytoplasmic shuttling. In the present work, we investigated the role of the nuclear factor kappa B (NFkappaB) and c-jun-N-terminal kinase (JNK), the functional hGR antagonists, in this process. We found that microtubule disarray caused by colchicine, vincristine or nocodazole does not activate NFkappaB in human hepatocytes as revealed by p50 and p65 subunits nuclear translocation. On the other hand, we demonstrate that JNK mediates hGR transcriptional inhibition by microtubules disarray, because a specific inhibitor of JNK, 1,9-pyrazoloanthrone (SP600125), partially blocked tyrosine aminotransferase mRNA suppression due to colchicine t
- The human glucocorticoid receptor (hGR) plays a pivotal role in cellular processes such as development, differentiation, homeostasis, immune response and in regulation of xenobiotic metabolism. It has been demonstrated recently that colchicine inhibits hGR transcriptional activity in primary cultures of human hepatocytes by a mechanism involving impairment of hGR nucleo-cytoplasmic shuttling. In the present work, we investigated the role of the nuclear factor kappa B (NFkappaB) and c-jun-N-terminal kinase (JNK), the functional hGR antagonists, in this process. We found that microtubule disarray caused by colchicine, vincristine or nocodazole does not activate NFkappaB in human hepatocytes as revealed by p50 and p65 subunits nuclear translocation. On the other hand, we demonstrate that JNK mediates hGR transcriptional inhibition by microtubules disarray, because a specific inhibitor of JNK, 1,9-pyrazoloanthrone (SP600125), partially blocked tyrosine aminotransferase mRNA suppression due to colchicine t (en)
- Lidský glukokortikoidní receptor (hGR) hraje stěžejní úlohu v buněčných procesech jakoje vývoj, diferenciace, homeostáza, imunitní odpověď a regulace metabolismu xenobiotik. Nedávno bylo prokázáno, že kolchicin inhibuje transkripční aktivitu hGR v primárních kulturách lidských hepatocytů mechanismem zahrnujícím narušení jaderné translokace hGR. V této práci jsme zkoumali úlohu jaderného faktoru kappa B NFkB a Lidský glukokortikoidní receptor (hGR) hraje stěžejní úlohu v buněčných procesech jakoje vývoj, diferenciace, homeostáza, imunitní odpověď a regulace metabolismu xenobiotik. Nedávno bylo prokázáno, že kolchicin inhibuje transkripční aktivitu hGR v primárních kulturách lidských hepatocytů mechanismem zahrnujícím narušení jaderné translokace hGR. V této práci jsme zkoumali úlohu jaderného faktoru kappa B NFkB a c-jun-N-terminální kinasy (JNK), funkčního antagonisty hGR, v tomto procesu. Zjistili jsme, že rozrušení mikrotubulů kolchicinem, vinkristinem nebo nokodazolem neaktivuje NFkB v lidských hep (cs)
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| Title
| - Microtubule disarray in primary cultures of human hepatocytes inhibits transcriptional activity of the glucocorticoid receptor via activation of c-jun N-terminal kinase
- Microtubule disarray in primary cultures of human hepatocytes inhibits transcriptional activity of the glucocorticoid receptor via activation of c-jun N-terminal kinase (en)
- Rozrušení mikrotubulů v primárních kulturách lidských hepatocytů inhibuje transkripční aktivitu glukokortikoidního receptoru prostřednictvím aktivace c-jun-N-terminální kinasy (cs)
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| skos:prefLabel
| - Microtubule disarray in primary cultures of human hepatocytes inhibits transcriptional activity of the glucocorticoid receptor via activation of c-jun N-terminal kinase
- Microtubule disarray in primary cultures of human hepatocytes inhibits transcriptional activity of the glucocorticoid receptor via activation of c-jun N-terminal kinase (en)
- Rozrušení mikrotubulů v primárních kulturách lidských hepatocytů inhibuje transkripční aktivitu glukokortikoidního receptoru prostřednictvím aktivace c-jun-N-terminální kinasy (cs)
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| skos:notation
| - RIV/61989592:15110/04:00001776!RIV06-MSM-15110___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GP303/04/P074), Z(MSM 151100003)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/04:00001776
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Microtubules; glucocorticoid receptor; c-jun N-terminal kinase; human hepatocytes; NFkB; colchicine (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Dvořák, Zdeněk
- Ulrichová, Jitka
- Modrianský, Martin
- Maurel, Patrick
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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