| Attributes | Values |
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| rdf:type
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| Description
| - COL); (2) zhodnotit účinek EIN na expresi a aktivitu cytochromu P450 (CYP). Primární lidské hepatocyty byly zvoleny jako model pro experimenty zaměřené na cytotoxicitu a expresi CYP. Uvolnění LDH a sekrece albuminu sloužily jako parametry cytotoxicity. EIN byl méně toxický než COL vzhledem k oběma parametrům a koncentracím v rozsahu 1-100 mikromolární. 10 mikromolární koncentrace EIN nezvyšovala expresi isoforem CYP1A2, CYP2A6, CYP2C9, CYP2C19, CYP2E1 a CYP3A4, které byly hodnoceny na úrovni mRNA, proteinu a specifické aktivity. EIN v koncentracích do 200 mikromolární neměl účinek na aktivity CYP1A2, 2C9, 2E1 a 3A4 v mikrosomální frakci lidských hepatocytů. Závěrem lze říci, že EIN v koncentracích do 10 mikromolární je netoxický v primárních lidských hepatocytech, jak prokázala sekrece albuminu a uvolnění LDH. Možné mezilékové interakce EIN díky účinkům na cytochromy P450 1A2, 2C9, 2E1 a 3A4 jsou nepravděpodobné, protože inhibiční a indukční studie žádné účinky neprokázaly. Jelikož EIN vykazuje lepší (cs)
- The aims of the present study were (1) to determine the cytotoxicity of colchiceine (EIN) in comparison with that of colchicine (COL); (2) to evaluate the effect of EIN on cytochrome P450 (CYP) expression and activity. Primary human hepatocytes were the model of choice for cytotoxicity and CYP expression experiments. LDH leakage and albumin secretion served as cytotoxicity parameters. EIN was less toxic than COL based on both parameters within the concentration range 1-100 microM. 10 microM concentration of EIN did not induce the expression of CYP1A2, CYP2A6, CYP2C9, CYP2C19, CYP2E1 and CYP3A4 isoforms, which were evaluated at the levels of mRNAs, proteins and specific activities in culture. EIN in concentrations up to 200 microM had no effect on marker activities of CYP1A2, 2C9, 2E1 and 3A4 in human liver microsomes. It was concluded that EIN in concentrations up to 10 microM is not cytotoxic in primary human hepatocytes as revealed by albumin secretion and LDH leakage. Possible drug-drug interaction
- The aims of the present study were (1) to determine the cytotoxicity of colchiceine (EIN) in comparison with that of colchicine (COL); (2) to evaluate the effect of EIN on cytochrome P450 (CYP) expression and activity. Primary human hepatocytes were the model of choice for cytotoxicity and CYP expression experiments. LDH leakage and albumin secretion served as cytotoxicity parameters. EIN was less toxic than COL based on both parameters within the concentration range 1-100 microM. 10 microM concentration of EIN did not induce the expression of CYP1A2, CYP2A6, CYP2C9, CYP2C19, CYP2E1 and CYP3A4 isoforms, which were evaluated at the levels of mRNAs, proteins and specific activities in culture. EIN in concentrations up to 200 microM had no effect on marker activities of CYP1A2, 2C9, 2E1 and 3A4 in human liver microsomes. It was concluded that EIN in concentrations up to 10 microM is not cytotoxic in primary human hepatocytes as revealed by albumin secretion and LDH leakage. Possible drug-drug interaction (en)
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| Title
| - Srovnání účinku kolchicinu a kolchiceinu na cytotoxicitu a expresi CYP genů v primárních kulturách lidských hepatocytů (cs)
- Comparative effect of colchicine and colchiceine on cytotoxicity and CYP gene expression in primary human hepatocytes
- Comparative effect of colchicine and colchiceine on cytotoxicity and CYP gene expression in primary human hepatocytes (en)
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| skos:prefLabel
| - Srovnání účinku kolchicinu a kolchiceinu na cytotoxicitu a expresi CYP genů v primárních kulturách lidských hepatocytů (cs)
- Comparative effect of colchicine and colchiceine on cytotoxicity and CYP gene expression in primary human hepatocytes
- Comparative effect of colchicine and colchiceine on cytotoxicity and CYP gene expression in primary human hepatocytes (en)
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| skos:notation
| - RIV/61989592:15110/02:00006985!RIV09-MSM-15110___
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| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GA303/99/P002), Z(MSM 151100003)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/02:00006985
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Colchiceine; colchicine; cytotoxicity; cytochrome P450; human hepatocyte (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Dvořák, Zdeněk
- Ulrichová, Jitka
- Modrianský, Martin
- Maurel, Patrick
- Pichard-Garcia, Lydiane
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| http://linked.open...n/vavai/riv/zamer
| |
| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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