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| Description
| - Effective scavenging of reactive radicals and low reactivity of generated secondary antioxidant radicals towards vital intracellular components are two critical requirements for a chain-breaking antioxidant. Tubulin-binding properties aside, colchicine metabolites remain largely untested for other possible biological activities, including antioxidant activity. Mourelle et al. [Life Sci. 45 (1989) 891] proposed that colchiceine (EIN) acts as an antioxidant and protective agent against lipid peroxidation in a rat model of liver injury. Since EIN as well as two other colchicine metabolites, 2-demethylcolchicine (2DM) and 3-demethylcolchicine (3DM), possess a hydroxy-group on their carbon ring that could participate in radical scavenging, we tested whether they can act as chain-breaking antioxidants. Using our fluorescence-HPLC assay with metabolically incorporated oxidation-sensitive cis-parinaric acid (PnA) we studied the effects of colchicine metabolites on peroxidation of different classes of membrane
- Effective scavenging of reactive radicals and low reactivity of generated secondary antioxidant radicals towards vital intracellular components are two critical requirements for a chain-breaking antioxidant. Tubulin-binding properties aside, colchicine metabolites remain largely untested for other possible biological activities, including antioxidant activity. Mourelle et al. [Life Sci. 45 (1989) 891] proposed that colchiceine (EIN) acts as an antioxidant and protective agent against lipid peroxidation in a rat model of liver injury. Since EIN as well as two other colchicine metabolites, 2-demethylcolchicine (2DM) and 3-demethylcolchicine (3DM), possess a hydroxy-group on their carbon ring that could participate in radical scavenging, we tested whether they can act as chain-breaking antioxidants. Using our fluorescence-HPLC assay with metabolically incorporated oxidation-sensitive cis-parinaric acid (PnA) we studied the effects of colchicine metabolites on peroxidation of different classes of membrane (en)
- Účinné vychytávání reaktivních radikálů a nízká reaktivita vzniklých sekundárních antioxidantů-radikálů vůči důležitým intracelulárním komponentám jsou dvě důležitá kritéria pro řetězec rušící antioxidant. Nehledě na vlastnosti vazby na tubulin, metabolity kolchicinu nebyly testovány na jiné biologické aktivity, včetně antioxidační aktivity. Protože kolchicein (EIN), stejně jako další dva metabolity kolchicinu, 2-demethylkolchicin (2-DM) a 3-demethylkolchicin (3-DM), mají hydroxy skupinu, která se může podílet na vychytávání radikálů, testovali jsme, zda mohou být řetězec rušícími antioxidanty. Žádný z testovaných metabolitů kolchicinu v rozsahu koncentrací od 1 do 100 mikromolární nebyl schopen chránit fofolipidy proti peroxidaci vyvolané buď azo-sloučeninami nebo reakcemi katalyzovanými myeloperoxidasou v přítomnosti peroxidu vodíku. Avšak metabolity vykazovaly koncentračně závislou depleci glutathionu, což připomíná chování etoposidu. Experimenty s elektronovou spin resonancí (ESR) demonstrovaly, ž (cs)
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| Title
| - Anti/pro-oxidační účinky fenolických sloučenin v buňkách: jsou metabolity kolchicinu řetězec rušícími antioxidanty? (cs)
- Anti-/pro-oxidant effects of phenolic compounds in cells; are colchicine metabolites chain-breaking antioxidants?
- Anti-/pro-oxidant effects of phenolic compounds in cells; are colchicine metabolites chain-breaking antioxidants? (en)
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| skos:prefLabel
| - Anti/pro-oxidační účinky fenolických sloučenin v buňkách: jsou metabolity kolchicinu řetězec rušícími antioxidanty? (cs)
- Anti-/pro-oxidant effects of phenolic compounds in cells; are colchicine metabolites chain-breaking antioxidants?
- Anti-/pro-oxidant effects of phenolic compounds in cells; are colchicine metabolites chain-breaking antioxidants? (en)
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| skos:notation
| - RIV/61989592:15110/02:00006970!RIV09-GA0-15110___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/02:00006970
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Lipid oxidation; antioxidant; radical scavenger; colchicine metabolites; parinaric acid; etoposide (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Modrianský, Martin
- Kagan, Valerian E.
- Matsura, Tatsuya
- Shvedova, Anna
- Tyurin, Vladimir
- Tyurina, Yulia
- Yalowich, Jack C.
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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