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| Description
| - Cryopreservation of porcine hepatocytes would ensure the accessibility of cells for laboratory use, permit the standardisation of experiments and save animals? lives. Therefore, in this study, we sought the optimal procedure for cryopreservation of porcine hepatocytes for both laboratory and clinical purposes. Hepatocytes were isolated from the liver lobe of a mini-pig by two-step collagenase perfusion. The cells were frozen with 20% foetal calf serum and 15% DMSO in two different media in four different concentrations ranging from 1 x 106 cells/ml to 5 x 106 cells/ml. 1.8 ml cryotubes and 120 ml Baxter bags were used for this purpose. Cells were cryopreserved either in a controlled freezer Sylab or step by step in a styrofoam box and stored at -196 ºC. The quality of fresh and cryopreserved hepatocytes were assessed by trypan blue exclusion test and by the evaluation of cytochrome P450 isoenzymes and glutathione-S-transferase activities; primary cultures were evaluated morphologically and by
- Cryopreservation of porcine hepatocytes would ensure the accessibility of cells for laboratory use, permit the standardisation of experiments and save animals? lives. Therefore, in this study, we sought the optimal procedure for cryopreservation of porcine hepatocytes for both laboratory and clinical purposes. Hepatocytes were isolated from the liver lobe of a mini-pig by two-step collagenase perfusion. The cells were frozen with 20% foetal calf serum and 15% DMSO in two different media in four different concentrations ranging from 1 x 106 cells/ml to 5 x 106 cells/ml. 1.8 ml cryotubes and 120 ml Baxter bags were used for this purpose. Cells were cryopreserved either in a controlled freezer Sylab or step by step in a styrofoam box and stored at -196 ºC. The quality of fresh and cryopreserved hepatocytes were assessed by trypan blue exclusion test and by the evaluation of cytochrome P450 isoenzymes and glutathione-S-transferase activities; primary cultures were evaluated morphologically and by (en)
- Kryoprezervace prasečích hepatocytů by zabezpečila dostupnost buněk pro laboratorní užití, dovolila standardizaci experimentů a ušetřila životy zvířat. V této práci jsme proto hledali nejlepší postup kryoprezervace prasečích hepatocytů jak pro laboratorní, tak pro klinické účely. Hepatocyty byly izolovány z jaterního laloku experimentálního miniaturního prasete pomocí dvoustupňové perfúze kolagenázou. Buňky byly poté zamraženy ve 20% fetálním telecím séru a 15% DMSO ve 2 různých médiích ve 4 koncentracích od 1 x 106 buněk/ml do 5 x 106 buněk/ml. Použity byly kryotuby o objemu 1.8 ml a vaky Baxter o objemu 120 ml. Buňky byly zamraženy buď řízeným mražením v přístroji Sylab, nebo postupným ochlazováním v polystyrénových boxech a poté uchovávány při -196 ºC. Kvalita čerstvých a kryoprezervovaných hepatocytů byla hodnocena v testu s trypanovou modří a měřením aktivity izoenzymů cytochromu P450 a glutathion-S-transferázy; primární kultury byly posuzovány morfologicky a testem MTT. Kryoprezervované h (cs)
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| Title
| - Optimizace kryoprezervace prasečích hepatocytů srovnáním životnosti a enzymatické aktivity čerstvých a kryoprezervovaných buněk (cs)
- Optimisation of porcine hepatocyte cryopreservation by comparison of viability and enzymatic activity of fresh and cryopreserved cells
- Optimisation of porcine hepatocyte cryopreservation by comparison of viability and enzymatic activity of fresh and cryopreserved cells (en)
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| skos:prefLabel
| - Optimizace kryoprezervace prasečích hepatocytů srovnáním životnosti a enzymatické aktivity čerstvých a kryoprezervovaných buněk (cs)
- Optimisation of porcine hepatocyte cryopreservation by comparison of viability and enzymatic activity of fresh and cryopreserved cells
- Optimisation of porcine hepatocyte cryopreservation by comparison of viability and enzymatic activity of fresh and cryopreserved cells (en)
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| skos:notation
| - RIV/61989592:15110/01:00006958!RIV09-MSM-15110___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA311/98/0648), Z(MSM 141100003), Z(MSM 151100003), Z(MZE-M03-99-01)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/01:00006958
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Freezing conditions; MTT test; cytochrom P450; primary cultures; porcine hepatocytes (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Bláha, Luděk
- Dvořák, Zdeněk
- Dítě, Petr
- Lata, Jan
- Ulrichová, Jitka
- Machala, Miroslav
- Šimánek, Vilím
- Smržová, Jana
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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