About: Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-? DNA sequences and 25S LSU rRNA sequences     Goto   Sponge   NotDistinct   Permalink

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  • The existence of two cryptic species within strains of the wood-decaying fungus Fomes fomentarius was revealed recently based on the internal transcribed spacer (ITS) sequence variability. In this study for the first time the sequences of another molecular markers, partial translation elongation factor 1-? (efa) region and partial 25S large subunit ribosomal RNA gene were obtained and used to evaluate genetic variability of F. fomentarius. Congruent phylogeny was observed for all three markers used confirming the presence of two cryptic species within F. fomentarius. Surprisingly, ITS sequence variability within F. fomentarius was significantly lower compared to the variability of efa sequences (0.023 versus 0.036 nucleotide substitutions per site) questioning the discriminatory power of ITS sequences for fungal species identification.
  • The existence of two cryptic species within strains of the wood-decaying fungus Fomes fomentarius was revealed recently based on the internal transcribed spacer (ITS) sequence variability. In this study for the first time the sequences of another molecular markers, partial translation elongation factor 1-? (efa) region and partial 25S large subunit ribosomal RNA gene were obtained and used to evaluate genetic variability of F. fomentarius. Congruent phylogeny was observed for all three markers used confirming the presence of two cryptic species within F. fomentarius. Surprisingly, ITS sequence variability within F. fomentarius was significantly lower compared to the variability of efa sequences (0.023 versus 0.036 nucleotide substitutions per site) questioning the discriminatory power of ITS sequences for fungal species identification. (en)
Title
  • Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-? DNA sequences and 25S LSU rRNA sequences
  • Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-? DNA sequences and 25S LSU rRNA sequences (en)
skos:prefLabel
  • Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-? DNA sequences and 25S LSU rRNA sequences
  • Genetic variability in Fomes fomentarius reconfirmed by translation elongation factor 1-? DNA sequences and 25S LSU rRNA sequences (en)
skos:notation
  • RIV/61988987:17310/13:A14018DZ!RIV14-MSM-17310___
http://linked.open...avai/riv/aktivita
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  • V
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  • 5
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  • 76398
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  • RIV/61988987:17310/13:A14018DZ
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  • Polyporaceae; cryptic species; Fomes fomentarius; molecular phylogeny; variability (en)
http://linked.open.../riv/klicoveSlovo
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  • SK - Slovenská republika
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  • [AF9477A5F7BA]
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  • BIOLOGIA
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  • 68
http://linked.open...iv/tvurceVysledku
  • Gáper, Ján
  • Judova, Jana
  • Pristas, Peter
  • Gaperova, Svetlana
http://linked.open...ain/vavai/riv/wos
  • 000323739200006
issn
  • 0006-3088
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  • 17310
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