About: Enhancement of p53 sequence-specific binding by DNA supercoiling

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About: Enhancement of p53 sequence-specific binding by DNA supercoiling Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Using a new competition assay, we investigated the effect of DNA negative supercoiling on the DNA sequence-specific binding ( SSDB) of human wild- type ( wt) p53 protein. We found that supercoiled ( sc) pBluescript DNAs with different inserted p53 target sequences were stronger competitors than a mixture of scDNA pBluescript with the given 20- mer target oligodeoxynucleotide. ScDNAs were always better competitors than their linearized or relaxed forms. Two DNAs with extruded cruciforms within the target sequence were the best competitors; removal of the cruciforms resulted in a decrease of competitor strength. In contrast to the full- length wt p53, the deletion mutant p53CDelta30 and the p53 core domain ( 93 - 312 aa) showed no enhancement of p53 SSDB to scDNA, suggesting that, in addition to the p53 core domain, the C- terminal was involved in this binding. Using a new competition assay, we investigated the effect of DNA negative supercoiling on the DNA sequence-specific binding ( SSDB) of human wild- type ( wt) p53 protein. We found that supercoiled ( sc) pBluescript DNAs with different inserted p53 target sequences were stronger competitors than a mixture of scDNA pBluescript with the given 20- mer target oligodeoxynucleotide. ScDNAs were always better competitors than their linearized or relaxed forms. Two DNAs with extruded cruciforms within the target sequence were the best competitors; removal of the cruciforms resulted in a decrease of competitor strength. In contrast to the full- length wt p53, the deletion mutant p53CDelta30 and the p53 core domain ( 93 - 312 aa) showed no enhancement of p53 SSDB to scDNA, suggesting that, in addition to the p53 core domain, the C- terminal was involved in this binding. (en)
Title	Enhancement of p53 sequence-specific binding by DNA supercoiling Enhancement of p53 sequence-specific binding by DNA supercoiling (en)
skos:prefLabel	Enhancement of p53 sequence-specific binding by DNA supercoiling Enhancement of p53 sequence-specific binding by DNA supercoiling (en)
skos:notation	RIV/61988987:17310/04:A1000V4D!RIV10-MSM-17310___
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(GA301/00/D001), P(IAA4004110), P(IAB5004203), P(OC D21.002), V, Z(AV0Z5004920)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Pečinka, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ostravská univerzita v Ostravě / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	562906
http://linked.open...ai/riv/idVysledku	RIV/61988987:17310/04:A1000V4D
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	competition assay; p53; DNA sequence-specific binding; supercoiled DNA; cruciform extrusion (en)
http://linked.open.../riv/klicoveSlovo	p53 DNA sequence-specific binding competition assay cruciform extrusion supercoiled DNA
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[142DB35D9AFD]
http://linked.open...i/riv/nazevZdroje	ONCOGENE
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20D21.002 Binding of human and mouse tumour suppressor protein p53 to linear and supercoiled DNAs Regulation of transcription factors binding to consensus sequences in superhelical DNA. Influence of phosphorylation of tumor supressor proteins Binding of tumor suppressor protein p53 to DNA. Effect of DNA supercoling and protein postsynthetic modification
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	23
http://linked.open...iv/tvurceVysledku	Brázda, Václav Brázdová, Marie Jagelská, Eva Paleček, Emil Pečinka, Petr Karlovská, Lenka
http://linked.open...n/vavai/riv/zamer	Biophysical properties of living systems and their changes under the influence of environmental factors
issn	0950-9232
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	17310

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