About: Expression and epigenetic profile of protoplast cultures (Cucumis sativus L.)     Goto   Sponge   NotDistinct   Permalink

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  • Cellular dedifferentiation is a process in which cells change from a given differentiated state into a transient, stem cell-like state and reenter into the cell cycle. In plants, cellular dedifferentiation is exemplified by protoplastization. Here, the cell wall of differentiated leaf cells is degraded by given enzymes that finish by protoplasts’ formation. The protoplasts assume a unique transient state with features resembling animal stem cells. The global chromatin decondensation is characterized for plant protoplasts that is also a distinguishing feature of animal stem cells. Various stimuli, that include phytohormones like auxins and cytokinins, induce protoplasts to reenter the cell cycle and proliferate. The reprogrammed cells could form calli, which under appropriate conditions can form shoots and roots, somatic embryos, and eventually fertile plant. However, application of auxins induces endoreduplication that could thwart future cell development; absence of these growth factors in culture of dedifferentiating protoplast cells causes their death with hallmark events of programmed cell death.
  • Cellular dedifferentiation is a process in which cells change from a given differentiated state into a transient, stem cell-like state and reenter into the cell cycle. In plants, cellular dedifferentiation is exemplified by protoplastization. Here, the cell wall of differentiated leaf cells is degraded by given enzymes that finish by protoplasts’ formation. The protoplasts assume a unique transient state with features resembling animal stem cells. The global chromatin decondensation is characterized for plant protoplasts that is also a distinguishing feature of animal stem cells. Various stimuli, that include phytohormones like auxins and cytokinins, induce protoplasts to reenter the cell cycle and proliferate. The reprogrammed cells could form calli, which under appropriate conditions can form shoots and roots, somatic embryos, and eventually fertile plant. However, application of auxins induces endoreduplication that could thwart future cell development; absence of these growth factors in culture of dedifferentiating protoplast cells causes their death with hallmark events of programmed cell death. (en)
Title
  • Expression and epigenetic profile of protoplast cultures (Cucumis sativus L.)
  • Expression and epigenetic profile of protoplast cultures (Cucumis sativus L.) (en)
skos:prefLabel
  • Expression and epigenetic profile of protoplast cultures (Cucumis sativus L.)
  • Expression and epigenetic profile of protoplast cultures (Cucumis sativus L.) (en)
skos:notation
  • RIV/61389030:_____/14:00441012!RIV15-AV0-61389030
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I
http://linked.open...iv/cisloPeriodika
  • 6
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  • 16149
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  • RIV/61389030:_____/14:00441012
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • SOMATIC EMBRYOGENESIS; DNA METHYLATION; CHROMATIN (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [411872CC5D55]
http://linked.open...i/riv/nazevZdroje
  • In Vitro Celluar and Developmental Biology - Plant
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 50
http://linked.open...iv/tvurceVysledku
  • Ondřej, V.
  • Cápal, Petr
http://linked.open...ain/vavai/riv/wos
  • 000346637300015
issn
  • 1054-5476
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s11627-014-9638-9
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