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  • Increased endogenous plant cytokinin (CK) content through transformation with an isopentyl transferase (ipt) gene has been associated with improved plant stress tolerance. The objective of this study is to determine amino acid changes associated with elevated CK production in ipt transgenic tobacco (Nicotiana tabacum L, cv. Wisconsin 38). Nontransformed (WT) and transformed tobacco plants with ipt gene controlled by senescence-activated promoter (SAG) were exposed to zinc soil contamination (tested levels Zn1=250, Zn2=500, Zn3=750 mg kg(-1) soil). The Zn effect on plant stress metabolism resulted in changes in levels of selected free amino acids playing an important role in adaptation to stress and plant senescence (alanine, leucine, proline, methionine and gamma-aminobutyrate) and differed for transformed and nontransformed tobacco plants. Analyses of amino adds confirmed that SAG tobacco plants had improved zinc tolerance compared with the WT plants. The enhanced Zn tolerance of SAG plants was associated with the maintenance of accumulation of proline, methionine and gamma-aminobutyrate. The concentrations of leucine and alanine did not show significant differences between plant lines.
  • Increased endogenous plant cytokinin (CK) content through transformation with an isopentyl transferase (ipt) gene has been associated with improved plant stress tolerance. The objective of this study is to determine amino acid changes associated with elevated CK production in ipt transgenic tobacco (Nicotiana tabacum L, cv. Wisconsin 38). Nontransformed (WT) and transformed tobacco plants with ipt gene controlled by senescence-activated promoter (SAG) were exposed to zinc soil contamination (tested levels Zn1=250, Zn2=500, Zn3=750 mg kg(-1) soil). The Zn effect on plant stress metabolism resulted in changes in levels of selected free amino acids playing an important role in adaptation to stress and plant senescence (alanine, leucine, proline, methionine and gamma-aminobutyrate) and differed for transformed and nontransformed tobacco plants. Analyses of amino adds confirmed that SAG tobacco plants had improved zinc tolerance compared with the WT plants. The enhanced Zn tolerance of SAG plants was associated with the maintenance of accumulation of proline, methionine and gamma-aminobutyrate. The concentrations of leucine and alanine did not show significant differences between plant lines. (en)
Title
  • The long-term effect of zinc soil contamination on selected free amino acids playing an important role in plant adaptation to stress and senescence
  • The long-term effect of zinc soil contamination on selected free amino acids playing an important role in plant adaptation to stress and senescence (en)
skos:prefLabel
  • The long-term effect of zinc soil contamination on selected free amino acids playing an important role in plant adaptation to stress and senescence
  • The long-term effect of zinc soil contamination on selected free amino acids playing an important role in plant adaptation to stress and senescence (en)
skos:notation
  • RIV/61389030:_____/14:00429992!RIV15-GA0-61389030
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • I, P(GAP501/11/1239)
http://linked.open...iv/cisloPeriodika
  • FEB 2014
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 26587
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/14:00429992
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Amino acid; Cytokinins; Senescence (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [FC83A2382B37]
http://linked.open...i/riv/nazevZdroje
  • Ecotoxicology and Enviromental Safety
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 100
http://linked.open...iv/tvurceVysledku
  • Procházková, Dagmar
  • Wilhelmová, Naděžda
  • Pavlík, Milan
  • Pavlíková, D.
  • Száková, J.
  • Zemanová, V.
http://linked.open...ain/vavai/riv/wos
  • 000330161000023
issn
  • 0147-6513
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.ecoenv.2013.10.028
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