About: Assaying nonspecific phospholipase C activity

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Plant nonspecific phospholipase C (NPC) is a recently described enzyme which plays a role in membrane rearrangement during phosphate starvation. It is also involved in responses of plants to brassinolide, abscisic acid (ABA), elicitors, and salt. The NPC activity is decreased in cells treated with aluminum. In the case of salt stress, the molecular mechanism of NPC action is based on accumulation of diacylglycerol (DAG) by hydrolysis of phospholipids and conversion of DAG, the product of NPC activity, to phosphatidic acid (PA) that participates in ABA signaling pathways. Here we describe a step-by-step protocol, which can be used to determine in situ or in vitro NPC activity. Determination is based on quantification of fluorescently labeled DAG as a product of cleavage of the fluorescently labeled substrate lipid, phosphatidylcholine. High-performance thin-layer chromatography is used for separation of fluorescent DAG. The spot is visualized with a laser scanner and the relative amounts of fluorescent DAG are quantified using imaging software. Plant nonspecific phospholipase C (NPC) is a recently described enzyme which plays a role in membrane rearrangement during phosphate starvation. It is also involved in responses of plants to brassinolide, abscisic acid (ABA), elicitors, and salt. The NPC activity is decreased in cells treated with aluminum. In the case of salt stress, the molecular mechanism of NPC action is based on accumulation of diacylglycerol (DAG) by hydrolysis of phospholipids and conversion of DAG, the product of NPC activity, to phosphatidic acid (PA) that participates in ABA signaling pathways. Here we describe a step-by-step protocol, which can be used to determine in situ or in vitro NPC activity. Determination is based on quantification of fluorescently labeled DAG as a product of cleavage of the fluorescently labeled substrate lipid, phosphatidylcholine. High-performance thin-layer chromatography is used for separation of fluorescent DAG. The spot is visualized with a laser scanner and the relative amounts of fluorescent DAG are quantified using imaging software. (en)
Title	Assaying nonspecific phospholipase C activity Assaying nonspecific phospholipase C activity (en)
skos:prefLabel	Assaying nonspecific phospholipase C activity Assaying nonspecific phospholipase C activity (en)
skos:notation	RIV/61389030:_____/13:00423956!RIV14-GA0-61389030
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GAP501/12/1942), P(GPP501/12/P950), Z(AV0Z50380511)
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Martinec, Jan Pejchar, Přemysl
http://linked.open.../riv/druhVysledku	C - Kapitola v knize
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	62213
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/13:00423956
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Bodipy; Diacylglycerol; Nonspecific phospholipase C (en)
http://linked.open.../riv/klicoveSlovo	Bodipy Diacylglycerol Nonspecific phospholipase C
http://linked.open...ontrolniKodProRIV	[D80BF93F3F2C]
http://linked.open...i/riv/mistoVydani	New York City
http://linked.open...i/riv/nazevZdroje	Plant Lipid Signaling Protocols
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...v/pocetStranKnihy	305 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Nonspecific phospholipase C: Molecular, cellular and functional characterisation of novel plant enzyme The role of diacylglycerol in aluminium toxicity in plants
http://linked.open...UplatneniVysledku	2013
http://linked.open...iv/tvurceVysledku	Martinec, Jan Pejchar, Přemysl Günther, F.E.S.
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/978-1-62703-401-2_18
http://purl.org/ne...btex#hasPublisher	Humana Press
https://schema.org/isbn	978-1-62703-400-5

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