About: Genetic fidelity in tissue-cultured 'Williams' bananas - The effect of high concentration of topolins and benzyladenine

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The effects of five topolins on genetic fidelity of micropropagated 'Williams' bananas were compared to that of 6-benzyladenine (BA). Sterile shoot-tip explants were cultured on modified Murashige and Skoog (MS) media supplemented with 30 mu M cytokinins (CKs) for 42 days. After the seventh subculture cycle, the degree of variability in regenerants was assessed using 10 inter simple sequence repeat (ISSR) markers. When compared to BA, the use of mTR and MemTTHP proved superior as they had a lower deleterious effect on the regenerants. However, the other topolins either produced a similar effect (MemTR) as BA or were even worse (mT). Inasmuch as the CK contribute to the degree of variation, the effect of subculture cycle remain prominent. Perhaps, the establishment of new culture lines after a few subculture cycles may mitigate the rate of variability. The current findings highlight the beneficial effect as well as the increasing importance of some of the topolins in alleviation of commonly observed physiological disorders during micropropagation. The effects of five topolins on genetic fidelity of micropropagated 'Williams' bananas were compared to that of 6-benzyladenine (BA). Sterile shoot-tip explants were cultured on modified Murashige and Skoog (MS) media supplemented with 30 mu M cytokinins (CKs) for 42 days. After the seventh subculture cycle, the degree of variability in regenerants was assessed using 10 inter simple sequence repeat (ISSR) markers. When compared to BA, the use of mTR and MemTTHP proved superior as they had a lower deleterious effect on the regenerants. However, the other topolins either produced a similar effect (MemTR) as BA or were even worse (mT). Inasmuch as the CK contribute to the degree of variation, the effect of subculture cycle remain prominent. Perhaps, the establishment of new culture lines after a few subculture cycles may mitigate the rate of variability. The current findings highlight the beneficial effect as well as the increasing importance of some of the topolins in alleviation of commonly observed physiological disorders during micropropagation. (en)
Title	Genetic fidelity in tissue-cultured 'Williams' bananas - The effect of high concentration of topolins and benzyladenine Genetic fidelity in tissue-cultured 'Williams' bananas - The effect of high concentration of topolins and benzyladenine (en)
skos:prefLabel	Genetic fidelity in tissue-cultured 'Williams' bananas - The effect of high concentration of topolins and benzyladenine Genetic fidelity in tissue-cultured 'Williams' bananas - The effect of high concentration of topolins and benzyladenine (en)
skos:notation	RIV/61389030:_____/13:00421037!RIV14-MZE-61389030
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(QI92A247), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	2013
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Doležal, Karel Szüčová, Lucie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	76387
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/13:00421037
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Cytokinins; Microsatellites; Musa spp. (en)
http://linked.open.../riv/klicoveSlovo	Cytokinins Microsatellites Musa spp.
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[5B7B36939110]
http://linked.open...i/riv/nazevZdroje	Scientia horticulturae
http://linked.open...in/vavai/riv/obor	GK
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Genetic diversity of elm relic populations in ed regions of the CR and their in vitro reproduction
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	161
http://linked.open...iv/tvurceVysledku	Doležal, Karel van Staden, J. Szüčová, Lucie Aremu, A. O. Bairu, M. W. Finnie, J. F.
http://linked.open...ain/vavai/riv/wos	000325595300047
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0304-4238
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.scienta.2013.07.022

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