About: Chlorophyll a fluorescence as a tool for a study of the Potato virus Y effects on photosynthesis of nontransgenic and transgenic Pssu-ipt tobacco     Goto   Sponge   NotDistinct   Permalink

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  • The effect of Potato virus Y (NTN) (PVY) infection upon photosynthesis was analysed in transgenic Pssu-ipt tobacco overproducing endogenous cytokinins in comparison with control, nontransgenic Nicotiana tabacum plants. The course of the infection from the early to the late stage was monitored by measuring of photosynthetic gas exchange and fast chlorophyll (Chl) a fluorescence induction kinetics. Leaf photosynthesis was also analysed using Chl fluorescence imaging (Chl-FI). From the different fluorescence parameters obtained using Chl-FI, the nonphotochemical quenching (NPQ) proved to be the most useful parameter to assess the effect of PVY infection. On the other hand, Chl-FI was found to be inapplicable for any presymptomatic detection of PVY infection in tobacco. The lower accumulation of the virus was found in transgenic plants and corresponded also with the presence of visible symptoms of PVY infection. The net photosynthetic rate (P (N)), transpiration rate (E), and stomatal conductance (g (s)) significantly decreased with the progress of the infection in both control plant types and transgenic rooted plants, while transgenic grafts were much less affected. The analysis of the Chl fluorescence transient revealed higher number of silent dissipative reaction centres, higher nonphotochemical dissipation, and significantly lower performance index, PI(abs), in the healthy transgenic grafts. Chl-FI also confirmed significantly higher NPQ in transgenic grafts.
  • The effect of Potato virus Y (NTN) (PVY) infection upon photosynthesis was analysed in transgenic Pssu-ipt tobacco overproducing endogenous cytokinins in comparison with control, nontransgenic Nicotiana tabacum plants. The course of the infection from the early to the late stage was monitored by measuring of photosynthetic gas exchange and fast chlorophyll (Chl) a fluorescence induction kinetics. Leaf photosynthesis was also analysed using Chl fluorescence imaging (Chl-FI). From the different fluorescence parameters obtained using Chl-FI, the nonphotochemical quenching (NPQ) proved to be the most useful parameter to assess the effect of PVY infection. On the other hand, Chl-FI was found to be inapplicable for any presymptomatic detection of PVY infection in tobacco. The lower accumulation of the virus was found in transgenic plants and corresponded also with the presence of visible symptoms of PVY infection. The net photosynthetic rate (P (N)), transpiration rate (E), and stomatal conductance (g (s)) significantly decreased with the progress of the infection in both control plant types and transgenic rooted plants, while transgenic grafts were much less affected. The analysis of the Chl fluorescence transient revealed higher number of silent dissipative reaction centres, higher nonphotochemical dissipation, and significantly lower performance index, PI(abs), in the healthy transgenic grafts. Chl-FI also confirmed significantly higher NPQ in transgenic grafts. (en)
Title
  • Chlorophyll a fluorescence as a tool for a study of the Potato virus Y effects on photosynthesis of nontransgenic and transgenic Pssu-ipt tobacco
  • Chlorophyll a fluorescence as a tool for a study of the Potato virus Y effects on photosynthesis of nontransgenic and transgenic Pssu-ipt tobacco (en)
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  • Chlorophyll a fluorescence as a tool for a study of the Potato virus Y effects on photosynthesis of nontransgenic and transgenic Pssu-ipt tobacco
  • Chlorophyll a fluorescence as a tool for a study of the Potato virus Y effects on photosynthesis of nontransgenic and transgenic Pssu-ipt tobacco (en)
skos:notation
  • RIV/61389030:_____/13:00396783!RIV14-AV0-61389030
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 65316
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/13:00396783
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • gas-exchange parameters; cytokinins; chlorophyll a fluorescence imaging (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [DEE89B555B40]
http://linked.open...i/riv/nazevZdroje
  • Photosynthetica
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 51
http://linked.open...iv/tvurceVysledku
  • Čeřovská, Noemi
  • Synková, Helena
  • Spoustová, Petra
  • Valcke, R.
http://linked.open...ain/vavai/riv/wos
  • 000318282600003
http://linked.open...n/vavai/riv/zamer
issn
  • 0300-3604
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s11099-013-0023-4
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