About: Role of Polyamines in Efficiency of Norway Spruce (Hurst Ecotype) Somatic Embryogenesis     Goto   Sponge   NotDistinct   Permalink

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Description
  • Micropropagation technologies represent a powerful tool for improvement and acceleration of tree breeding programs in forestry. Tissue culture approaches, in particular somatic embryogenesis, hold considerable promise for breeding programs of coniferous trees. Utilization of somatic embryogenesis could facilitate reproduction of rare or selected coniferous genotypes. Despite the availability of a successful protocol for generating Norway spruce plants using a somatic embryogenesis technique, there is a lack of data concerning the endogenous composition of biologically active compounds in somatic embryos. Beside the key roles of auxins and cytokinins, very important function in differentiation processes belongs to polyamines although the mechanism of their action is still not fully cleared. Positive correlation between embryogenic capacity and total content of free PAs confirmed crucial role of PAs during somatic embryo development. It was shown that the predominant PAs in somatic embryos of highly responsible Hurst ecotype of Norway spruce (L10) was spermidine, while embryos of less responsible ecotype (L13, L16, L17 and L28) contained lower level of Spd and/or inadequate Put/Spd ratio. Exogenously supplied PAs might therefore affect and improve the induction and somatic embryo development in less responsible plant genotypes. The studies related to the improving of somatic embryogenesis method in less responsible Hurst ecotype of Norway spruce are now in progress in our laboratory.
  • Micropropagation technologies represent a powerful tool for improvement and acceleration of tree breeding programs in forestry. Tissue culture approaches, in particular somatic embryogenesis, hold considerable promise for breeding programs of coniferous trees. Utilization of somatic embryogenesis could facilitate reproduction of rare or selected coniferous genotypes. Despite the availability of a successful protocol for generating Norway spruce plants using a somatic embryogenesis technique, there is a lack of data concerning the endogenous composition of biologically active compounds in somatic embryos. Beside the key roles of auxins and cytokinins, very important function in differentiation processes belongs to polyamines although the mechanism of their action is still not fully cleared. Positive correlation between embryogenic capacity and total content of free PAs confirmed crucial role of PAs during somatic embryo development. It was shown that the predominant PAs in somatic embryos of highly responsible Hurst ecotype of Norway spruce (L10) was spermidine, while embryos of less responsible ecotype (L13, L16, L17 and L28) contained lower level of Spd and/or inadequate Put/Spd ratio. Exogenously supplied PAs might therefore affect and improve the induction and somatic embryo development in less responsible plant genotypes. The studies related to the improving of somatic embryogenesis method in less responsible Hurst ecotype of Norway spruce are now in progress in our laboratory. (en)
Title
  • Role of Polyamines in Efficiency of Norway Spruce (Hurst Ecotype) Somatic Embryogenesis
  • Role of Polyamines in Efficiency of Norway Spruce (Hurst Ecotype) Somatic Embryogenesis (en)
skos:prefLabel
  • Role of Polyamines in Efficiency of Norway Spruce (Hurst Ecotype) Somatic Embryogenesis
  • Role of Polyamines in Efficiency of Norway Spruce (Hurst Ecotype) Somatic Embryogenesis (en)
skos:notation
  • RIV/61389030:_____/12:00390495!RIV13-AV0-61389030
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(QH82303), Z(AV0Z50380511)
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
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  • 166044
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  • RIV/61389030:_____/12:00390495
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  • Norway Spruce; Somatic Embryogenesis; Polyamines (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...ontrolniKodProRIV
  • [402A8C973370]
http://linked.open...i/riv/mistoVydani
  • Rijeka
http://linked.open...i/riv/nazevZdroje
  • Embryogenesis
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...v/pocetStranKnihy
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...iv/tvurceVysledku
  • Cvikrová, Milena
  • Malá, J.
  • Máchová, P.
  • Gemperlová, Lenka
http://linked.open...n/vavai/riv/zamer
number of pages
http://bibframe.org/vocab/doi
  • 10.5772/2143
http://purl.org/ne...btex#hasPublisher
  • InTech
https://schema.org/isbn
  • 978-953-51-0466-7
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