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  • Genome analysis in many plant species is hampered by large genome size and by sequence redundancy due to the presence of repetitive DNA and polyploidy. One solution is to reduce the sample complexity by dissecting the genomes to single chromosomes. This can be realized by flow cytometric sorting, which enables purification of chromosomes in large numbers. Coupling the chromosome sorting technology with next generation sequencing provides a targeted and cost effective way to tackle complex genomes. The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing.
  • Genome analysis in many plant species is hampered by large genome size and by sequence redundancy due to the presence of repetitive DNA and polyploidy. One solution is to reduce the sample complexity by dissecting the genomes to single chromosomes. This can be realized by flow cytometric sorting, which enables purification of chromosomes in large numbers. Coupling the chromosome sorting technology with next generation sequencing provides a targeted and cost effective way to tackle complex genomes. The methods outlined in this article describe a procedure for preparation of chromosomal DNA suitable for next-generation sequencing. (en)
Title
  • Flow cytometric chromosome sorting in plants: The next generation
  • Flow cytometric chromosome sorting in plants: The next generation (en)
skos:prefLabel
  • Flow cytometric chromosome sorting in plants: The next generation
  • Flow cytometric chromosome sorting in plants: The next generation (en)
skos:notation
  • RIV/61389030:_____/12:00381153!RIV13-AV0-61389030
http://linked.open...avai/riv/aktivita
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  • P(GAP501/10/1740), Z(AV0Z50380511)
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  • 3
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  • 136827
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  • RIV/61389030:_____/12:00381153
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  • Chromosome sorting; Flow cytometry; Fluorescence in situ hybridization (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [2DA843F49310]
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  • Methods
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  • 57
http://linked.open...iv/tvurceVysledku
  • Doležel, Jaroslav
  • Kubaláková, Marie
  • Číhalíková, Jarmila
  • Šimková, Hana
  • Vrána, Jan
http://linked.open...ain/vavai/riv/wos
  • 000308852400009
http://linked.open...n/vavai/riv/zamer
issn
  • 1046-2023
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.ymeth.2012.03.006
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