About: Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants

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About: Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Transient expression of foreign genes based on plant viral vectors is a suitable system for the production of immunogens that can be used for development of a new generation of vaccines against a variety of infectious diseases. In this study the epitope derived from HPV-16 L2 minor capsid protein (amino acids 108–120) was expressed from Potato virus X (PVX)-based vector pGR106 as N- or C-terminal fusion with the PVX coat protein (PVX CP) in transgenic Nicotiana benthamiana plants. The protein L2108-120-PVX CP was expressed in plants at a level of 170 mg/kg of fresh leaf tissue. The protein PVX CP-L2108-120 was expressed using mutated vector to avoid homologous recombination at a level of 8 mg/kg of fresh leaf tissue. Immunogenicity of L2108-120-PVX CP virus-like particles was tested after immunization of mice by subcutaneous injection or tattoo administration. In animal sera the antibodies against the PVX CP and the L2108-120 epitope were found after both methods of vaccine delivery. Transient expression of foreign genes based on plant viral vectors is a suitable system for the production of immunogens that can be used for development of a new generation of vaccines against a variety of infectious diseases. In this study the epitope derived from HPV-16 L2 minor capsid protein (amino acids 108–120) was expressed from Potato virus X (PVX)-based vector pGR106 as N- or C-terminal fusion with the PVX coat protein (PVX CP) in transgenic Nicotiana benthamiana plants. The protein L2108-120-PVX CP was expressed in plants at a level of 170 mg/kg of fresh leaf tissue. The protein PVX CP-L2108-120 was expressed using mutated vector to avoid homologous recombination at a level of 8 mg/kg of fresh leaf tissue. Immunogenicity of L2108-120-PVX CP virus-like particles was tested after immunization of mice by subcutaneous injection or tattoo administration. In animal sera the antibodies against the PVX CP and the L2108-120 epitope were found after both methods of vaccine delivery. (en)
Title	Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants (en)
skos:prefLabel	Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants Transient expression of Human papillomavirus type 16 L2 epitope fused to N- and C-terminus of coat protein of Potato virus X in plants (en)
skos:notation	RIV/61389030:_____/12:00380685!RIV13-AV0-61389030
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GA521/06/0973), P(GA521/09/1525), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Štorchová, Helena Čeřovská, Noemi Folwarczna, Jitka Moravec, Tomáš Hoffmeisterová, Hana Plchová, Helena
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	174914
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/12:00380685
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Human papillomavirus (HPV-16); L2-and E7-derived epitopes; transient expression (en)
http://linked.open.../riv/klicoveSlovo	Human papillomavirus (HPV-16) L2-and E7-derived epitopes transient expression
http://linked.open...odStatuVydavatele	IN - Indická republika
http://linked.open...ontrolniKodProRIV	[FA36BBF22C79]
http://linked.open...i/riv/nazevZdroje	Journal of Biosciences
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	Expression of papillomavirus antigenic epitopes in plants using plant viral vector Biosafe plant virus expression system for transient production of human papillomavirus oncoproteins and their use for therapeutic vaccine development
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	37
http://linked.open...iv/tvurceVysledku	Moravec, Tomáš Folwarczna, Jitka Hoffmeisterová, Hana Plchová, Helena Ryšlavá, H. Čeřovská, Noemi Ludvíková, V. Synková, Helena Šmahel, M.
http://linked.open...ain/vavai/riv/wos	000301163400015
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0250-5991
number of pages	9 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s12038-011-9177-z

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