About: Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://home.ueb.cas.cz/publikace/2012_Navratil_IOBC_61-67.pdf
Description	Extracellular serine proteases are often used as a factor of pathogenicity. To improve potato resistance to the pathogens, we prepared plants containing a gene derived from the wax moth Galleria mellonella and expressing the silk protease inhibitor 2 (SPI2). The synthetic SPI2 gene was fused with mGFP-5 to facilitate its detection in plants. Cultivar Velox, which exhibits medium resistance to Phytophthora infestans, was used for transgenosis. The polyclonal antibody confirmed the presence of a protein of expected size in the extracts of transgenic plants. The presence of the fusion protein in resistant plants was confirmed with additional analytical methods. Indirect ELISA showed up to 3 fold increase of protease inhibition in transformed plants compared to the control background level. The tested resistance to late blight showed heterogeneity among transgenic lines, i.e. some lines were more resistant and others were more susceptible than the non-transgenic plants. Extracellular serine proteases are often used as a factor of pathogenicity. To improve potato resistance to the pathogens, we prepared plants containing a gene derived from the wax moth Galleria mellonella and expressing the silk protease inhibitor 2 (SPI2). The synthetic SPI2 gene was fused with mGFP-5 to facilitate its detection in plants. Cultivar Velox, which exhibits medium resistance to Phytophthora infestans, was used for transgenosis. The polyclonal antibody confirmed the presence of a protein of expected size in the extracts of transgenic plants. The presence of the fusion protein in resistant plants was confirmed with additional analytical methods. Indirect ELISA showed up to 3 fold increase of protease inhibition in transformed plants compared to the control background level. The tested resistance to late blight showed heterogeneity among transgenic lines, i.e. some lines were more resistant and others were more susceptible than the non-transgenic plants. (en)
Title	Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack (en)
skos:prefLabel	Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack Protease inhibitors as a possible new factor in agricultural plant protection against microbial and fungal attack (en)
skos:notation	RIV/61389030:_____/12:00380582!RIV13-AV0-61389030
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1M06030), P(QI91A229), Z(AV0Z50070508), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	Jan 2012
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Navrátil, Oldřich
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	163030
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/12:00380582
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	potato; transgenosis; microbial and fungal pathogens (en)
http://linked.open.../riv/klicoveSlovo	potato transgenosis microbial and fungal pathogens
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[6DA518C95EC6]
http://linked.open...i/riv/nazevZdroje	IOBC-Wprs Bulletin
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional genomics and proteomics for crop improvement Application of conventional and molecular genetic approaches for the development of grain legumes resistant to viral and fungal pathogens and insect pests.
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	73
http://linked.open...iv/tvurceVysledku	Horáčková, V. Kodrík, Dalibor Sehnal, František Navrátil, Oldřich Vinokurov, Konstantin Kludkiewicz, Barbara
http://linked.open...n/vavai/riv/zamer	Study of the regulation of insect organism, dynamics of insect populations and function of insects in ecosystems Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	1027-3115
number of pages	7 (xsd:int)

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software