About: Enrichment techniques employed in phosphoproteomics

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Rapid changes of protein phosphorylation play a crucial role in the regulation of many cellular processes. Being post-translationally modified, phosphoproteins are often present in quite low abundance and tend to co-exist with their unphosphorylated isoform within the cell. To make their identification more practicable, the use of enrichment protocols is often required. The enrichment strategies can be performed either at the level of phosphoproteins or at the level of phosphopeptides. Both approaches have their advantages and disadvantages. Most enriching strategies are based on chemical modifications, affinity chromatography to capture peptides and proteins containing negatively charged phosphate groups onto a positively charged matrix, or immunoprecipitation by phospho-specific antibodies. In this article, the most up-to-date enrichment techniques are discussed, taking into account their optimization, and highlighting their advantages and disadvantages. Rapid changes of protein phosphorylation play a crucial role in the regulation of many cellular processes. Being post-translationally modified, phosphoproteins are often present in quite low abundance and tend to co-exist with their unphosphorylated isoform within the cell. To make their identification more practicable, the use of enrichment protocols is often required. The enrichment strategies can be performed either at the level of phosphoproteins or at the level of phosphopeptides. Both approaches have their advantages and disadvantages. Most enriching strategies are based on chemical modifications, affinity chromatography to capture peptides and proteins containing negatively charged phosphate groups onto a positively charged matrix, or immunoprecipitation by phospho-specific antibodies. In this article, the most up-to-date enrichment techniques are discussed, taking into account their optimization, and highlighting their advantages and disadvantages. (en)
Title	Enrichment techniques employed in phosphoproteomics Enrichment techniques employed in phosphoproteomics (en)
skos:prefLabel	Enrichment techniques employed in phosphoproteomics Enrichment techniques employed in phosphoproteomics (en)
skos:notation	RIV/61389030:_____/12:00366394!RIV13-AV0-61389030
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/09/0858), P(GA525/09/0994), P(GAP501/11/1462), P(OC08011), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2013
http://linked.open...aciTvurceVysledku	Honys, David Fíla, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	134462
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/12:00366394
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Phosphoproteomics; Enrichment; IMAC (en)
http://linked.open.../riv/klicoveSlovo	Enrichment Phosphoproteomics IMAC
http://linked.open...odStatuVydavatele	AT - Rakouská republika
http://linked.open...ontrolniKodProRIV	[4F7AD194C763]
http://linked.open...i/riv/nazevZdroje	Amino Acids
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Integrative analysis of the role of bZIP transcription factors in pollen maturation Chemotaxonomy of isoflavonoids Functional characterisation of pollen unique storage ribonucleoprotein particles Importance of nucleoprotein complexes for the regulation of the male gametophyte development
http://linked.open...UplatneniVysledku	2012
http://linked.open...v/svazekPeriodika	43
http://linked.open...iv/tvurceVysledku	Fíla, Jan Honys, David
http://linked.open...ain/vavai/riv/wos	000307536100003
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0939-4451
number of pages	23 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s00726-011-1111-z

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