About: Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns

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About: Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Mature pollen grain represents a highly desiccated structure with an extremely tough cell wall. Thanks to it, it resists common proteomic protocols. Instead, a robust homogenization has to be performed since proteins are needed to burst out of the cell to be included in the extracted proteome fraction. Here, a novel way of pollen homogenization employing Roche MagNA Lyser Instrument is presented, sparing time and laborious work. However, plant proteomics does not rely solely on perfect homogenization; also the choice of the extraction protocol is of key importance. The composition of the extraction buffer has a decisive influence on which proteome fraction will be extracted. Therefore the second part of our study is dedicated to the comparison of different extraction protocols with respect to subsequent proteomic analyses. Mature pollen grain represents a highly desiccated structure with an extremely tough cell wall. Thanks to it, it resists common proteomic protocols. Instead, a robust homogenization has to be performed since proteins are needed to burst out of the cell to be included in the extracted proteome fraction. Here, a novel way of pollen homogenization employing Roche MagNA Lyser Instrument is presented, sparing time and laborious work. However, plant proteomics does not rely solely on perfect homogenization; also the choice of the extraction protocol is of key importance. The composition of the extraction buffer has a decisive influence on which proteome fraction will be extracted. Therefore the second part of our study is dedicated to the comparison of different extraction protocols with respect to subsequent proteomic analyses. (en)
Title	Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns (en)
skos:prefLabel	Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns Impact of homogenization and protein extraction conditions on the obtained tobacco pollen proteomic patterns (en)
skos:notation	RIV/61389030:_____/11:00366403!RIV12-AV0-61389030
http://linked.open...avai/riv/aktivita	I P Z
http://linked.open...avai/riv/aktivity	I, P(GAP501/11/1462), P(OC08011), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Feciková, Jana Honys, David Čapková, Věra Fíla, Jan
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	203673
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/11:00366403
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	proteomics; Roche MagNA Lyser Instrument; ARABIDOPSIS-THALIANA (en)
http://linked.open.../riv/klicoveSlovo	ARABIDOPSIS-THALIANA proteomics Roche MagNA Lyser Instrument
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[6249DDD21648]
http://linked.open...i/riv/nazevZdroje	Biologia Plantarum
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Functional characterisation of pollen unique storage ribonucleoprotein particles Importance of nucleoprotein complexes for the regulation of the male gametophyte development
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	55
http://linked.open...iv/tvurceVysledku	Feciková, Jana Fíla, Jan Honys, David Čapková, Věra
http://linked.open...ain/vavai/riv/wos	000293029800013
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0006-3134
number of pages	8 (xsd:int)
http://bibframe.org/vocab/doi	10.1007/s10535-011-0116-5

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