About: First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Wheat is one of the world’s most important crops and is characterized by a large polyploid genome. One way to reduce genome complexity is to isolate single chromosomes using flow cytometry. Low coverage DNA sequencing can provide a snapshot of individual chromosomes, allowing a fast characterization of their main features and comparison with other genomes. We used massively parallel 454 pyrosequencing to obtain a 2x coverage of wheat chromosome 5A. The resulting sequence assembly was used to identify TEs, genes and miRNAs, as well as to infer a virtual gene order based on the synteny with other grass genomes. Repetitive elements account for more than 75% of the genome. Gene content was estimated considering non-redundant reads showing at least one match to ESTs or proteins. The results indicate that the coding fraction represents 1.08% and 1.3% of the short and long arm respectively, projecting the number of genes of the whole chromosome to approximately 5,000. Wheat is one of the world’s most important crops and is characterized by a large polyploid genome. One way to reduce genome complexity is to isolate single chromosomes using flow cytometry. Low coverage DNA sequencing can provide a snapshot of individual chromosomes, allowing a fast characterization of their main features and comparison with other genomes. We used massively parallel 454 pyrosequencing to obtain a 2x coverage of wheat chromosome 5A. The resulting sequence assembly was used to identify TEs, genes and miRNAs, as well as to infer a virtual gene order based on the synteny with other grass genomes. Repetitive elements account for more than 75% of the genome. Gene content was estimated considering non-redundant reads showing at least one match to ESTs or proteins. The results indicate that the coding fraction represents 1.08% and 1.3% of the short and long arm respectively, projecting the number of genes of the whole chromosome to approximately 5,000. (en)
Title	First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach (en)
skos:prefLabel	First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach First Survey of the Wheat Chromosome 5A Composition through a Next Generation Sequencing Approach (en)
skos:notation	RIV/61389030:_____/11:00366036!RIV12-AV0-61389030
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0007/01/01), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	10
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Doležel, Jaroslav Šimková, Hana Kubaláková, Marie
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	199863
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/11:00366036
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	wheat chromosome 5A; Chromosome arm sorting; DNA amplification (en)
http://linked.open.../riv/klicoveSlovo	Chromosome arm sorting wheat chromosome 5A DNA amplification
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[30735D9D62C7]
http://linked.open...i/riv/nazevZdroje	PLoS ONE
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	21 (xsd:int)
http://linked.open...vavai/riv/projekt	Centre of the Region Haná for Biotechnological and Agricultural Research
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	6
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Kubaláková, Marie Šimková, Hana Cattivelli, L. Albiero, A. Forcato, C. Perrotta, G. Vitulo, N.
http://linked.open...ain/vavai/riv/wos	000296186900060
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	1932-6203
number of pages	14 (xsd:int)
http://bibframe.org/vocab/doi	10.1371/journal.pone.0026421

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