About: BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character. BAC libraries represent an essential tool for positional cloning. However, wheat BAC libraries comprise more than milion clones, which makes thein screening very laborious. Here, we present a targeted approach based on chromosome-specific BAC libraries. Such libraries were constructed from flow-sorted arms of wheat chromosome 7D. A library from the short arm (7DS) consisting of 49,152 clones with 113 kb insert size represented 12.1 arm equivalents whereas a library from the long arm (7DL) comprised 50,304 clones of 116 kb providing 14.9x arm coverage. The 7DS library was PCR screened with markers linked to Russian wheat aphid resistance gene DnCI2401, the 7DL library was screened by hybridization with a probe linked to greenbug resistence gene Gb3. The small number of clones combined with high coverage made the screening highly efficient and cost effective. Positional cloning in bread wheat is a tedious task due to its huge genome size and hexaploid character. BAC libraries represent an essential tool for positional cloning. However, wheat BAC libraries comprise more than milion clones, which makes thein screening very laborious. Here, we present a targeted approach based on chromosome-specific BAC libraries. Such libraries were constructed from flow-sorted arms of wheat chromosome 7D. A library from the short arm (7DS) consisting of 49,152 clones with 113 kb insert size represented 12.1 arm equivalents whereas a library from the long arm (7DL) comprised 50,304 clones of 116 kb providing 14.9x arm coverage. The 7DS library was PCR screened with markers linked to Russian wheat aphid resistance gene DnCI2401, the 7DL library was screened by hybridization with a probe linked to greenbug resistence gene Gb3. The small number of clones combined with high coverage made the screening highly efficient and cost effective. (en)
Title	BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes (en)
skos:prefLabel	BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes BAC Libraries from Wheat Chromosome 7D: Efficient Tool for Positional Cloning of Aphid Resistance Genes (en)
skos:notation	RIV/61389030:_____/11:00364122!RIV12-AV0-61389030
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA521/07/1573), P(LC06004), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	302543
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	Robert-Quatre, Heda Šafář, Jan Bartoš, Jan Doležel, Jaroslav Šimková, Hana Kubaláková, Marie Suchánková, Pavla Číhalíková, Jarmila
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	187732
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/11:00364122
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	TRITICUM-AESTIVUM L.; HEXAPLOID WHEAT; BREAD WHEAT (en)
http://linked.open.../riv/klicoveSlovo	BREAD WHEAT HEXAPLOID WHEAT TRITICUM-AESTIVUM L.
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[0133F79E5561]
http://linked.open...i/riv/nazevZdroje	Journal of Biomedicine and Biotechnology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	8 (xsd:int)
http://linked.open...cetTvurcuVysledku	15 (xsd:int)
http://linked.open...vavai/riv/projekt	Creation of resources for wheat genomics and map-based cloning of resistance genes from chromosome 7D Integration of research activities to study the plant genome
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	-
http://linked.open...iv/tvurceVysledku	Azhaguvel, P. Bartoš, Jan Doležel, Jaroslav Kubaláková, Marie Lapitan, N. L. V. Luo, M. C. Ma, Y. Q. Peng, J. Robert-Quatre, Heda Suchánková, Pavla Weng, Y. Q. You, F. M. Šafář, Jan Číhalíková, Jarmila Šimková, Hana
http://linked.open...ain/vavai/riv/wos	000286232600001
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	1110-7243
number of pages	11 (xsd:int)
http://bibframe.org/vocab/doi	10.1155/2011/302543

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 48 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software