About: Cdk2 Inhibition Prolongs G1 Phase Progression in Mouse Embryonic Stem Cells

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Embryonic stem cells (ESCs) proliferate rapidly and have a unique cell-cycle structure with a very short G1 phase. Previous reports suggested that the rapid G1 phase progression of ESCs might be underpinned by high and precocious Cdk2 activity and that Cdk2 activity might be crucial for both cell-cycle regulation and cell-fate decisions in human ESCs. However, the actual role of Cdk2 in cell-cycle progression of mouse ESCs (mESCs) has not been elucidated. In this study, we investigated the effects of down-regulation of Cdk2 activity by olomoucine II in 2 mESC lines. Olomoucine II treatment signifi cantly increased the G1 phase cell numbers, decreased the S phase cell numbers, and inhibited DNA replication in mESCs. In nocodazole-synchronized mESCs, we show that specifi c down-regulation of Cdk2 activity prolongs G1 phase progression. In addition, down-regulation of Cdk2 activity in mESCs established a somatic cell-like cell cycle and induced expression of differentiation markers. Embryonic stem cells (ESCs) proliferate rapidly and have a unique cell-cycle structure with a very short G1 phase. Previous reports suggested that the rapid G1 phase progression of ESCs might be underpinned by high and precocious Cdk2 activity and that Cdk2 activity might be crucial for both cell-cycle regulation and cell-fate decisions in human ESCs. However, the actual role of Cdk2 in cell-cycle progression of mouse ESCs (mESCs) has not been elucidated. In this study, we investigated the effects of down-regulation of Cdk2 activity by olomoucine II in 2 mESC lines. Olomoucine II treatment signifi cantly increased the G1 phase cell numbers, decreased the S phase cell numbers, and inhibited DNA replication in mESCs. In nocodazole-synchronized mESCs, we show that specifi c down-regulation of Cdk2 activity prolongs G1 phase progression. In addition, down-regulation of Cdk2 activity in mESCs established a somatic cell-like cell cycle and induced expression of differentiation markers. (en)
Title	Cdk2 Inhibition Prolongs G1 Phase Progression in Mouse Embryonic Stem Cells Cdk2 Inhibition Prolongs G1 Phase Progression in Mouse Embryonic Stem Cells (en)
skos:prefLabel	Cdk2 Inhibition Prolongs G1 Phase Progression in Mouse Embryonic Stem Cells Cdk2 Inhibition Prolongs G1 Phase Progression in Mouse Embryonic Stem Cells (en)
skos:notation	RIV/61389030:_____/10:00359295!RIV11-GA0-61389030
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA301/08/1649), P(NR9508), Z(MSM6198959205), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Kryštof, Vladimír
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	249787
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/10:00359295
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	embryonic stem cells; cell cycle; G1 phase (en)
http://linked.open.../riv/klicoveSlovo	embryonic stem cells G1 phase cell cycle
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[E0319635ED2B]
http://linked.open...i/riv/nazevZdroje	Stem Cells and Development
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Activation of Chk1 and Chk2 kinases in response to stress in mouse embryonic stem cells. Modulation of cell division of normal and cancer cells by cyclin-dependent kinase inhibitors
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	19
http://linked.open...iv/tvurceVysledku	Kryštof, Vladimír Divoký, V. Doležel, P. Calábková, L. Koledová, Z. Rašková-Kafková, L.
http://linked.open...ain/vavai/riv/wos	000274929400005
http://linked.open...n/vavai/riv/zamer	Studies of genes and molecular mechanisms involved in regulation of hematopoiesis, their clinical impact and targeted treatment Modulation of signalling and regulatory pathways in normal and cancer cells
issn	1547-3287
number of pages	13 (xsd:int)

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