About: An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins

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About: An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A set of 11 relevant isoprenoid cytokinins, including zeatin isomers, was separated by ultra performance liquid chromatography in less than 6 min. The iP-type cytokinins were observed to give rise to a previously-unknown fragment at m/z 69; we suggest that the diagnostic (204-69) transition can be used to monitor the biosynthetic rate of isopentenyladenine. Furthermore, we found that by treating the cytokinin nucleotides with alkaline phosphatase prior to analysis, the sensitivity of the detection process could be increased. In addition, derivatization (propionylation) improved the ESIMS response by increasing the analytes' hydrophobicity. Indeed, the ESI-MS response of propionylated isopentenyladenosine was about 34% higher than that of its underivatized counterpart. Moreover, the response of the derivatized zeatin ribosides was about 75% higher than that of underivatized zeatin ribosides. Finally, we created a web-based calculator (IZOTOP) that facilitates MS/MS data processing. A set of 11 relevant isoprenoid cytokinins, including zeatin isomers, was separated by ultra performance liquid chromatography in less than 6 min. The iP-type cytokinins were observed to give rise to a previously-unknown fragment at m/z 69; we suggest that the diagnostic (204-69) transition can be used to monitor the biosynthetic rate of isopentenyladenine. Furthermore, we found that by treating the cytokinin nucleotides with alkaline phosphatase prior to analysis, the sensitivity of the detection process could be increased. In addition, derivatization (propionylation) improved the ESIMS response by increasing the analytes' hydrophobicity. Indeed, the ESI-MS response of propionylated isopentenyladenosine was about 34% higher than that of its underivatized counterpart. Moreover, the response of the derivatized zeatin ribosides was about 75% higher than that of underivatized zeatin ribosides. Finally, we created a web-based calculator (IZOTOP) that facilitates MS/MS data processing. (en)
Title	An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins (en)
skos:prefLabel	An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins An improved in vivo deuterium labeling method for measuring the biosynthetic rate of cytokinins (en)
skos:notation	RIV/61389030:_____/10:00359292!RIV11-GA0-61389030
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(ED0007/01/01), P(ED0017/01/01), P(GA301/08/1649), P(GA522/08/0920), Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Doležal, Karel Novák, Ondřej Frébortová, Jitka Tarkowski, Petr Václavíková, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	246231
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/10:00359292
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cytokinin; deuterium labelling; biosynthetic rate (en)
http://linked.open.../riv/klicoveSlovo	cytokinin biosynthetic rate deuterium labelling
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[1113FCAF3F6E]
http://linked.open...i/riv/nazevZdroje	Molecules
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	APPLICATION LABORATORIES OF ADVANCED MICROTECHNOLOGIES AND NANOTECHNOLOGIES Effect of synthetic cytokinin analogues on plant hormone perception, metabolism and phenotypic alterations Centre of the Region Haná for Biotechnological and Agricultural Research Modulation of cell division of normal and cancer cells by cyclin-dependent kinase inhibitors
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	15
http://linked.open...iv/tvurceVysledku	Doležal, Karel Novák, Ondřej Šebela, M. Frébortová, Jitka Tarkowski, Petr Floková, K. Václavíková, Kateřina Jaworek, P. Nordström, A. Raus, M.
http://linked.open...ain/vavai/riv/wos	000285709000046
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Modulation of signalling and regulatory pathways in normal and cancer cells
issn	1420-3049
number of pages	16 (xsd:int)

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