About: The role of actin isoforms in somatic embryogenesis in Norway spruce

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Application of low doses of latrunculin B (lat B) during the maturation of somatic embryos predominantly killed suspensor cells while leaving the cells in meristematic centres alive, indicating differential sensitivity of actin in the two cell types. The treatment resulted in faster development of advanced embryos into mature somatic embryos and elimination of insufficiently developed ones. We analysed the composition of actin isoforms in the culture and isolated four spruce actin genes. Analysis of their expression during embryo maturation revealed that one actin isoform was expressed constitutively in both cell types, whereas three actin isoforms were expressed predominantly in suspensor cells and their expression declined during the maturation. The expression decline was enhanced by Lat B treatment. Sequence analysis revealed amino-acid substitutions in the Lat B-binding site in one of the suspensor-specific actin isoforms, which may result in a different binding affinity for Lat B. Application of low doses of latrunculin B (lat B) during the maturation of somatic embryos predominantly killed suspensor cells while leaving the cells in meristematic centres alive, indicating differential sensitivity of actin in the two cell types. The treatment resulted in faster development of advanced embryos into mature somatic embryos and elimination of insufficiently developed ones. We analysed the composition of actin isoforms in the culture and isolated four spruce actin genes. Analysis of their expression during embryo maturation revealed that one actin isoform was expressed constitutively in both cell types, whereas three actin isoforms were expressed predominantly in suspensor cells and their expression declined during the maturation. The expression decline was enhanced by Lat B treatment. Sequence analysis revealed amino-acid substitutions in the Lat B-binding site in one of the suspensor-specific actin isoforms, which may result in a different binding affinity for Lat B. (en)
Title	The role of actin isoforms in somatic embryogenesis in Norway spruce The role of actin isoforms in somatic embryogenesis in Norway spruce (en)
skos:prefLabel	The role of actin isoforms in somatic embryogenesis in Norway spruce The role of actin isoforms in somatic embryogenesis in Norway spruce (en)
skos:notation	RIV/61389030:_____/10:00356952!RIV11-MSM-61389030
http://linked.open...avai/riv/aktivita	P R S Z
http://linked.open...avai/riv/aktivity	P(LC06034), P(ME 668), P(OC 158), R, S, Z(AV0Z50380511), Z(MSM0021620858)
http://linked.open...iv/cisloPeriodika	89
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Vágner, Martin Vondráková, Zuzana Eliášová, Kateřina
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	285674
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/10:00356952
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	PROGRAMMED CELL-DEATH; GENE FAMILY; F-ACTIN (en)
http://linked.open.../riv/klicoveSlovo	PROGRAMMED CELL-DEATH F-ACTIN GENE FAMILY
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[E61BE5C2BEA3]
http://linked.open...i/riv/nazevZdroje	BMC Plant Biology
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	10 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/OC%20158 Regulation of morphogenesis of plant cells and organs http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20668
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	10
http://linked.open...iv/tvurceVysledku	Fischer, L. Vágner, Martin Eliášová, Kateřina Vondráková, Zuzana Opatrný, Z. Schwarzerová, K. Bellinvia, E. Boříková, P. Fišerová, J. Havelková, L.
http://linked.open...ain/vavai/riv/wos	000279956500001
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Signaling and cellular response mechanisms
issn	1471-2229
number of pages	13 (xsd:int)

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