About: Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants     Goto   Sponge   NotDistinct   Permalink

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  • Cytokinin analogue roscovitine exhibits a strong inhibitory effect on cytokinin N-glucosylation. Roscovitine-resistant mutant of Arabidopsis thaliana (L.) Heynh. (ror-1) was isolated using T-DNA tagged lines, in order to find a gene involved in cytokinin N-glucosylation. The amount of cytokinin N-glucosides of trans-zeatin- and isopentenyladenine-type was elevated by 20% in ror-1 mutant compared to WT. The cytokinin oxidase/dehydrogenase activity exhibited a mild elevation in ror-1 compared to WT in basal media. The ror-1 plants showed slightly enhanced resistance to exogenous aromatic cytokinins (benzyladenine). Incubation with 5 µM BA for 24 h resulted in significant up-regulation of ROR-1 gene expression in ror-1 mutant. In silico analysis showed that ROR-1 gene encoded for a protein consisting of GRAM (Glycosyltransferases Rab-like GTPase activators and Myotubularins) and C2 domains. Here, we report on the role of ROR-1 gene in metabolism of bioactive cytokinins in the plants.
  • Cytokinin analogue roscovitine exhibits a strong inhibitory effect on cytokinin N-glucosylation. Roscovitine-resistant mutant of Arabidopsis thaliana (L.) Heynh. (ror-1) was isolated using T-DNA tagged lines, in order to find a gene involved in cytokinin N-glucosylation. The amount of cytokinin N-glucosides of trans-zeatin- and isopentenyladenine-type was elevated by 20% in ror-1 mutant compared to WT. The cytokinin oxidase/dehydrogenase activity exhibited a mild elevation in ror-1 compared to WT in basal media. The ror-1 plants showed slightly enhanced resistance to exogenous aromatic cytokinins (benzyladenine). Incubation with 5 µM BA for 24 h resulted in significant up-regulation of ROR-1 gene expression in ror-1 mutant. In silico analysis showed that ROR-1 gene encoded for a protein consisting of GRAM (Glycosyltransferases Rab-like GTPase activators and Myotubularins) and C2 domains. Here, we report on the role of ROR-1 gene in metabolism of bioactive cytokinins in the plants. (en)
Title
  • Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants
  • Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants (en)
skos:prefLabel
  • Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants
  • Characterization of Arabidopsis thaliana mutant ror-1 (roscovitine-resistant) and its utilization in understanding of the role of cytokinin N-glucosylation pathway in plants (en)
skos:notation
  • RIV/61389030:_____/10:00350379!RIV11-MSM-61389030
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(IAA600380701), P(LC06034), P(ME 505), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 250414
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/10:00350379
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Cytokinin metabolism; N-Glucosyltransferase; GRAM domain (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [B5125F6C9474]
http://linked.open...i/riv/nazevZdroje
  • Plant Growth Regulation
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 61
http://linked.open...iv/tvurceVysledku
  • Motyka, Václav
  • Vaňková, Radomíra
  • Žižková, Eva
  • Herrera, C.
  • Auer, C.
  • Dwivedi, S.
http://linked.open...ain/vavai/riv/wos
  • 000278411500003
http://linked.open...n/vavai/riv/zamer
issn
  • 0167-6903
number of pages
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