About: Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The large bread wheat genome (1C similar to 17 Gbp) contains a preponderance of repetitive DNA and the species is polyploid. These characteristics together serve to hamper the molecular analysis of the wheat genome. Its complexity can, however, be reduced by using flow cytometry to isolate individual chromosomes, and these can be exploited to construct chromosome-specific BAC libraries. Such libraries simplify the task of physical map construction, positional cloning and the targeted development of genetic markers. Rapid improvements in the efficiency and cost of DNA sequencing provide an opportunity to contemplate sequencing the wheat genome by preparing sequence-ready physical maps for each chromosome or chromosome arm in turn. The quality of the chromosome-specific libraries depends on their chromosome coverage and the mean insert size. First-generation libraries suffered from a relatively low mean insert size. The large bread wheat genome (1C similar to 17 Gbp) contains a preponderance of repetitive DNA and the species is polyploid. These characteristics together serve to hamper the molecular analysis of the wheat genome. Its complexity can, however, be reduced by using flow cytometry to isolate individual chromosomes, and these can be exploited to construct chromosome-specific BAC libraries. Such libraries simplify the task of physical map construction, positional cloning and the targeted development of genetic markers. Rapid improvements in the efficiency and cost of DNA sequencing provide an opportunity to contemplate sequencing the wheat genome by preparing sequence-ready physical maps for each chromosome or chromosome arm in turn. The quality of the chromosome-specific libraries depends on their chromosome coverage and the mean insert size. First-generation libraries suffered from a relatively low mean insert size. (en)
Title	Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat (en)
skos:prefLabel	Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat Development of Chromosome-Specific BAC Resources for Genomics of Bread Wheat (en)
skos:notation	RIV/61389030:_____/10:00349181!RIV11-GA0-61389030
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA521/07/1573), P(LC06004), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	1-3
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Šafář, Jan Bartoš, Jan Doležel, Jaroslav Šimková, Hana Kubaláková, Marie Suchánková, Pavla Číhalíková, Jarmila
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	253931
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/10:00349181
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	BAC library; Chromosome; DNA markers (en)
http://linked.open.../riv/klicoveSlovo	Chromosome DNA markers BAC library
http://linked.open...odStatuVydavatele	CH - Švýcarská konfederace
http://linked.open...ontrolniKodProRIV	[FF6ADC6761F5]
http://linked.open...i/riv/nazevZdroje	Cytogenetics and Genome Research
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Creation of resources for wheat genomics and map-based cloning of resistance genes from chromosome 7D Integration of research activities to study the plant genome
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	129
http://linked.open...iv/tvurceVysledku	Bartoš, Jan Doležel, Jaroslav Kubaláková, Marie Suchánková, Pavla Šafář, Jan Číhalíková, Jarmila Šimková, Hana
http://linked.open...ain/vavai/riv/wos	000280519500023
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	1424-8581
number of pages	13 (xsd:int)

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