About: Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species

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About: Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The presence of closely related genomes in polyploid species makes the assembly of total genomic sequence from shotgun sequence reads produced by the current sequencing platforms exceedingly difficult, if not impossible. Genomes of polyploid species could be sequenced following the ordered clone sequencing approach employing contigs of bacterial artificial chromosome (BAC) clones and BAC-based physical maps. In this work, physical mapping utilizing the SNaPshot HICF of BAC libraries of allohexaploid common wheat in which it is possible to construct single-chromosome or single-chromosome-arm BAC libraries from DNA of flow-sorted chromosomes and bypass the obstacles created by polyploidy. Physical maps of equally good BAC contigs, specific for three wheat single-chromosome libraries (3B, 3AS and 3DS), were constructed in this work. Because of the high purity of the resulting assembled contigs, they can be directly used for genome sequencing. The presence of closely related genomes in polyploid species makes the assembly of total genomic sequence from shotgun sequence reads produced by the current sequencing platforms exceedingly difficult, if not impossible. Genomes of polyploid species could be sequenced following the ordered clone sequencing approach employing contigs of bacterial artificial chromosome (BAC) clones and BAC-based physical maps. In this work, physical mapping utilizing the SNaPshot HICF of BAC libraries of allohexaploid common wheat in which it is possible to construct single-chromosome or single-chromosome-arm BAC libraries from DNA of flow-sorted chromosomes and bypass the obstacles created by polyploidy. Physical maps of equally good BAC contigs, specific for three wheat single-chromosome libraries (3B, 3AS and 3DS), were constructed in this work. Because of the high purity of the resulting assembled contigs, they can be directly used for genome sequencing. (en)
Title	Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species (en)
skos:prefLabel	Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species Feasibility of physical map construction from fingerprinted bacterial artificial chromosome libraries of polyploid plant species (en)
skos:notation	RIV/61389030:_____/10:00347998!RIV11-AV0-61389030
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	122
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Šafář, Jan Doležel, Jaroslav Šimková, Hana Kopecký, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	258910
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/10:00347998
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	BREAD WHEAT; TETRAPLOID WHEAT; GENETIC-MAP (en)
http://linked.open.../riv/klicoveSlovo	BREAD WHEAT GENETIC-MAP TETRAPLOID WHEAT
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[279DF2B5470B]
http://linked.open...i/riv/nazevZdroje	B M C Genomics
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	11
http://linked.open...iv/tvurceVysledku	Doležel, Jaroslav Luo, M. C. Ma, Y. Q. You, F. M. Šafář, Jan Šimková, Hana Kopecký, David Anderson, O. D. Dvorak, J. Gill, B. McGuire, P. E.
http://linked.open...ain/vavai/riv/wos	000275628900001
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	1471-2164
number of pages	8 (xsd:int)

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