About: Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries

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About: Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	In bread wheat comprehensive genome analysis is complicated by its very large genome (17 Gbp), polyploid nature and high repetitive sequence content. Using flow cytometry allows dissecting the wheat genome to individual chromosomes. Flow sorted chromosomes were used already to construct several grain BAC libraries including those from chromosome 3B and chromosome arms 1BS and 1RS. In the present work we compare the construction of the 3AS/3DS BAC libraries in respect to our new improved protocol which made two-sized selection feasible. A minimal tiling path of chromosome 3B has already been established. Construction of the 3AS- and 3DS-specific BAC libraries represent significant step toward completing BAC resources for the homoeologous chromosome group 3 of wheat and accelerate the development of sequence-ready physical contig maps and gene cloning, as well as comparative analyses aiming at revealing the genome changes accompanying the evolution of homoeologous chromosome group 3. In bread wheat comprehensive genome analysis is complicated by its very large genome (17 Gbp), polyploid nature and high repetitive sequence content. Using flow cytometry allows dissecting the wheat genome to individual chromosomes. Flow sorted chromosomes were used already to construct several grain BAC libraries including those from chromosome 3B and chromosome arms 1BS and 1RS. In the present work we compare the construction of the 3AS/3DS BAC libraries in respect to our new improved protocol which made two-sized selection feasible. A minimal tiling path of chromosome 3B has already been established. Construction of the 3AS- and 3DS-specific BAC libraries represent significant step toward completing BAC resources for the homoeologous chromosome group 3 of wheat and accelerate the development of sequence-ready physical contig maps and gene cloning, as well as comparative analyses aiming at revealing the genome changes accompanying the evolution of homoeologous chromosome group 3. (en)
Title	Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries (en)
skos:prefLabel	Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries (en)
skos:notation	RIV/61389030:_____/09:00337706!RIV10-MSM-61389030
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA521/06/1723), P(GA521/07/1573), P(GD521/05/H013), P(LC06004), P(OC08025), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Šafář, Jan Bartoš, Jan Doležel, Jaroslav Šimková, Hana Kubaláková, Marie Suchánková, Pavla Číhalíková, Jarmila
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	316091
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/09:00337706
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	BAC library; Flow sorting; Homoeologous chromosomes (en)
http://linked.open.../riv/klicoveSlovo	Flow sorting Homoeologous chromosomes BAC library
http://linked.open...odStatuVydavatele	IT - Italská republika
http://linked.open...ontrolniKodProRIV	[CA8051619B07]
http://linked.open...i/riv/nazevZdroje	Journal of Genetics & Breeding
http://linked.open...in/vavai/riv/obor	GE
http://linked.open...ichTvurcuVysledku	7 (xsd:int)
http://linked.open...cetTvurcuVysledku	11 (xsd:int)
http://linked.open...vavai/riv/projekt	Creation of resources for wheat genomics and map-based cloning of resistance genes from chromosome 7D Integration of research activities to study the plant genome Construction of physical map of the hexaploid wheat D genome using the chromosome-based approach Construction of BAC library and physical mapping of the wheat chromosome 3D Wheat - from genom to products quality
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	61
http://linked.open...iv/tvurceVysledku	Bartoš, Jan Doležel, Jaroslav Kubaláková, Marie Suchánková, Pavla Šafář, Jan Číhalíková, Jarmila Šimková, Hana Gill, B. S. Lucretti, S. Fiocchetti, F. Roselli, M.
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0394-9257
number of pages	10 (xsd:int)

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