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rdf:type
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Description
| - In bread wheat comprehensive genome analysis is complicated by its very large genome (17 Gbp), polyploid nature and high repetitive sequence content. Using flow cytometry allows dissecting the wheat genome to individual chromosomes. Flow sorted chromosomes were used already to construct several grain BAC libraries including those from chromosome 3B and chromosome arms 1BS and 1RS. In the present work we compare the construction of the 3AS/3DS BAC libraries in respect to our new improved protocol which made two-sized selection feasible. A minimal tiling path of chromosome 3B has already been established. Construction of the 3AS- and 3DS-specific BAC libraries represent significant step toward completing BAC resources for the homoeologous chromosome group 3 of wheat and accelerate the development of sequence-ready physical contig maps and gene cloning, as well as comparative analyses aiming at revealing the genome changes accompanying the evolution of homoeologous chromosome group 3.
- In bread wheat comprehensive genome analysis is complicated by its very large genome (17 Gbp), polyploid nature and high repetitive sequence content. Using flow cytometry allows dissecting the wheat genome to individual chromosomes. Flow sorted chromosomes were used already to construct several grain BAC libraries including those from chromosome 3B and chromosome arms 1BS and 1RS. In the present work we compare the construction of the 3AS/3DS BAC libraries in respect to our new improved protocol which made two-sized selection feasible. A minimal tiling path of chromosome 3B has already been established. Construction of the 3AS- and 3DS-specific BAC libraries represent significant step toward completing BAC resources for the homoeologous chromosome group 3 of wheat and accelerate the development of sequence-ready physical contig maps and gene cloning, as well as comparative analyses aiming at revealing the genome changes accompanying the evolution of homoeologous chromosome group 3. (en)
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Title
| - Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries
- Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries (en)
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skos:prefLabel
| - Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries
- Generating resources for genomics of wheat homoeologous chromosome group 3: 3AS- and 3DS-specific BAC libraries (en)
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skos:notation
| - RIV/61389030:_____/09:00337706!RIV10-MSM-61389030
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA521/06/1723), P(GA521/07/1573), P(GD521/05/H013), P(LC06004), P(OC08025), Z(AV0Z50380511)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/09:00337706
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - BAC library; Flow sorting; Homoeologous chromosomes (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Genetics & Breeding
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Bartoš, Jan
- Doležel, Jaroslav
- Kubaláková, Marie
- Suchánková, Pavla
- Šafář, Jan
- Číhalíková, Jarmila
- Šimková, Hana
- Gill, B. S.
- Lucretti, S.
- Fiocchetti, F.
- Roselli, M.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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