About: AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis     Goto   Sponge   NotDistinct   Permalink

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  • Two SOB five-like (SOFL) genes, AtSOFL1 and AtSOFL2, were characterized in Arabidopsis (Arabidopsis thaliana). They act redundantly in regulating specific cytokinin levels. Analysis of the translational fusion AtSOFL1:AtSOFL1-GUS and AtSOFL2:AtSOFL2-GUS indicated that AtSOFL1 and AtSOFL2 are expressed predominantly in vascular tissues of developing leaves, flowers and siliques, but barely detectable in roots and stems. Overexpression of either AtSOFL1 or AtSOFL2 led to increased cytokinin content and corresponding mutant phenotypes for both transgenic seedlings and adult plants. SOFL domain was proved necessary for AtSOFL2´s overexpression phenotypes. Silencing or disrupting either of them caused no obvious developmental defects. The SOFL1-RNAi62/sofl2-1 double mutant accumulated lower levels of trans-zeatin riboside monophosphate (tZRMP) and N6-(2-isopentenyl)adenosine monophosphate (iPRMP), precursors of bioactive cytokinins, and displayed decreased response to exogenous cytokinin.
  • Two SOB five-like (SOFL) genes, AtSOFL1 and AtSOFL2, were characterized in Arabidopsis (Arabidopsis thaliana). They act redundantly in regulating specific cytokinin levels. Analysis of the translational fusion AtSOFL1:AtSOFL1-GUS and AtSOFL2:AtSOFL2-GUS indicated that AtSOFL1 and AtSOFL2 are expressed predominantly in vascular tissues of developing leaves, flowers and siliques, but barely detectable in roots and stems. Overexpression of either AtSOFL1 or AtSOFL2 led to increased cytokinin content and corresponding mutant phenotypes for both transgenic seedlings and adult plants. SOFL domain was proved necessary for AtSOFL2´s overexpression phenotypes. Silencing or disrupting either of them caused no obvious developmental defects. The SOFL1-RNAi62/sofl2-1 double mutant accumulated lower levels of trans-zeatin riboside monophosphate (tZRMP) and N6-(2-isopentenyl)adenosine monophosphate (iPRMP), precursors of bioactive cytokinins, and displayed decreased response to exogenous cytokinin. (en)
Title
  • AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis
  • AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis (en)
skos:prefLabel
  • AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis
  • AtSOFL1 and AtSOFL2 Act Redundantly as Positive Modulators of the Endogenous Content of Specific Cytokinins in Arabidopsis (en)
skos:notation
  • RIV/61389030:_____/09:00337486!RIV10-AV0-61389030
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA206/06/1306), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika
  • 12
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 304362
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/09:00337486
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • TRANSGENIC TOBACCO PLANTS; OXIDASE/DEHYDROGENASE GENES; ISOPENTENYL TRANSFERASE (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [68563725624B]
http://linked.open...i/riv/nazevZdroje
  • PLoS ONE
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 4
http://linked.open...iv/tvurceVysledku
  • Dobrev, Petre
  • Malbeck, Jiří
  • Vaňková, Radomíra
  • Zhang, J.
  • Xu, Y.
  • Chong, K.
  • Neff, M. M.
http://linked.open...ain/vavai/riv/wos
  • 000272829800020
http://linked.open...n/vavai/riv/zamer
issn
  • 1932-6203
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