About: Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter

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About: Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The cytokinin biosynthesis gene, isopentenyl transferase (ipt), under the control of a 821 bp fragment of the LEACO1 gene promoter (from Lycopersicon esculentum) was introduced into Dendranthema x grandiflorium Iridon’ (chrysanthemum). LEACO10.821kb-ipt transgenic lines exhibited in the vegetative state enhanced branching and reduced internode lengths. In the generative state, transformed lines exhibited elevated flower bud counts (3.8 – 6.7x more than WT), which was associated with a delay of flower bud development and a decrease in flower bud diameter. RT-PCR analysis indicated differences in ipt gene expression between individual transgenic lines, as well as at different stages of generative shoot development. Expression of ipt in transgenic lines correlated well with high concentrations of endogenous cytokinins. In general, transgenic lines accumulated more cytokinins, including cytokinin O-glucosides and N-glucosides in generative shoots than in the vegetative ones. The cytokinin biosynthesis gene, isopentenyl transferase (ipt), under the control of a 821 bp fragment of the LEACO1 gene promoter (from Lycopersicon esculentum) was introduced into Dendranthema x grandiflorium Iridon’ (chrysanthemum). LEACO10.821kb-ipt transgenic lines exhibited in the vegetative state enhanced branching and reduced internode lengths. In the generative state, transformed lines exhibited elevated flower bud counts (3.8 – 6.7x more than WT), which was associated with a delay of flower bud development and a decrease in flower bud diameter. RT-PCR analysis indicated differences in ipt gene expression between individual transgenic lines, as well as at different stages of generative shoot development. Expression of ipt in transgenic lines correlated well with high concentrations of endogenous cytokinins. In general, transgenic lines accumulated more cytokinins, including cytokinin O-glucosides and N-glucosides in generative shoots than in the vegetative ones. (en)
Title	Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter (en)
skos:prefLabel	Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter Enhancement of flowering and branching phenotype in chrysanthemum by expression of ipt under the control of a 0.821 kb fragment of the LEACO1 gene promoter (en)
skos:notation	RIV/61389030:_____/09:00337485!RIV10-AV0-61389030
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Vaňková, Radomíra Malbeck, Jiří
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	313289
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/09:00337485
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	ipt Gene; Cytokinins; Transgenic chrysanthemum (en)
http://linked.open.../riv/klicoveSlovo	Cytokinins Transgenic chrysanthemum ipt Gene
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[0542EFCA4DBE]
http://linked.open...i/riv/nazevZdroje	Plant Cell Reports
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	28
http://linked.open...iv/tvurceVysledku	Malbeck, Jiří Vaňková, Radomíra Li, Y. Khodakovskaya, M. Li, A. McAvoy, R.
http://linked.open...ain/vavai/riv/wos	000269208100006
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0721-7714
number of pages	12 (xsd:int)

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