| Attributes | Values |
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| rdf:type
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| Description
| - Průtoková cytometrie umožňuje třídění chromozómů, které mohou být využity při analýze genomu. Získání mikrogramových množství DNA vyžaduje vytřídění miliónů chromozómů, což je pracné a časově náročné. Mnoho genomických aplikací, jako je vývoj genetických map nebo fyzické mapování, však nevyžaduje dlouhé fragmenty DNA. V takových případech může být doba třídění minimalizovaná využitím celogenomové amplifikace. V této práci je popsán protokol pro amplifikaci DNA z deseti tisíc chromozómů ječmene, které mohou být vytříděny za méně než jednu hodinu. Srování přítomnosti SNP markerů v amplifikované a neamplikované DNA potvrdilo vysokou reprezentativnost amplifikace. Tato metoda podstatně rozšiřuje možnosti využití tříděných chromozómů v genomice rostlin. (cs)
- Background: Flow cytometry facilitates sorting of single chromosomes and chromosome arms which can be used for targeted genome analysis. However, the recovery of microgram amounts of DNA needed for some assays requires sorting of millions of chromosomes which is laborious and time consuming. Yet, many genomic applications such as development of genetic maps or physical mapping do not require large DNA fragments. In such cases time-consuming de novo sorting can be minimized by utilizing whole-genome amplification. Results: Here we report a protocol optimized in barley including amplification of DNA from only ten thousand chromosomes, which can be isolated in less than one hour. Flow-sorted chromosomes were treated with proteinase K and amplified using Phi29 multiple displacement amplification (MDA). Overnight amplification in a 20-microlitre reaction produced 3.7 – 5.7 micrograms DNA with a majority of products between 5 and 30 kb. To determine the purity of sorted fractions and potential amplifica...
- Background: Flow cytometry facilitates sorting of single chromosomes and chromosome arms which can be used for targeted genome analysis. However, the recovery of microgram amounts of DNA needed for some assays requires sorting of millions of chromosomes which is laborious and time consuming. Yet, many genomic applications such as development of genetic maps or physical mapping do not require large DNA fragments. In such cases time-consuming de novo sorting can be minimized by utilizing whole-genome amplification. Results: Here we report a protocol optimized in barley including amplification of DNA from only ten thousand chromosomes, which can be isolated in less than one hour. Flow-sorted chromosomes were treated with proteinase K and amplified using Phi29 multiple displacement amplification (MDA). Overnight amplification in a 20-microlitre reaction produced 3.7 – 5.7 micrograms DNA with a majority of products between 5 and 30 kb. To determine the purity of sorted fractions and potential amplifica... (en)
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| Title
| - Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley
- Využití DNA amplifikované z tříděných chromozómů pro mapování SNA markerů u ječmene (cs)
- Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley (en)
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| skos:prefLabel
| - Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley
- Využití DNA amplifikované z tříděných chromozómů pro mapování SNA markerů u ječmene (cs)
- Coupling amplified DNA from flow-sorted chromosomes to high-density SNP mapping in barley (en)
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| skos:notation
| - RIV/61389030:_____/08:00322569!RIV09-AV0-61389030
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(GD521/05/H013), P(LC06004), P(ME 884), Z(AV0Z50380511)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/08:00322569
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - Flow cytometry; DNA amplification; Hordeum vulgare L. (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Bartoš, Jan
- Bhat, P. R.
- Close, T. J.
- Doležel, Jaroslav
- Hřibová, Eva
- Suchánková, Pavla
- Šafář, Jan
- Šimková, Hana
- Condamine, P.
- Svensson, J. T.
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| http://linked.open...ain/vavai/riv/wos
| |
| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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