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  • Studium hladin endogenních cytokininů a popis jejich kolísání v časovém průběhu ukázaly na možnou roli cytokininů během klíčení a ujmutí semenáčků Pisum sativum L. Kořeny byly rozděleny na různé časti a v těch byly stanovovány endogenní cytokininy. Celkový obsah cytokininů se zvyšoval a ribotidy byly převládající formou. Byla studována i biosyntéza isopentenyladeninu a zeatinu. Kolísání koncentrací u různych forem cytokininů ukazuje, že v kořenech hrachu mohou být syntetizovány z různých drah. (cs)
  • Germination and seedling establishment follows a distinct pattern which is partly controlled by hormones. Roots have high levels of cytokinins. By quantifying the fluctuations in endogenous cytokinins over time, further insight may be gained into the role of cytokinins during germination and seedling establishment. Radicles were excised from sterile Pisum sativum L. seeds after 30 min and 5 h imbibition. Seedlings germinated on agar were harvested after 1, 3, 6 and 9 days. The roots were divided into the root tip, root free zone, secondary root zone and from day 6, the secondary roots. Samples were purified by various chromatographic methods and endogenous cytokinins detected by LC(+)ES-MS. Benzyladenine levels doubled after 5 h imbibition and then gradually decreased over time. Low concentrations of cis-Zeatin (cZ) type cytokinins were detected in the radicle after 30 min imbibition. After 5 h imbibition, cis-zeatin riboside-5'-monophosphate had greatly increased. The total cytokinin content of th...
  • Germination and seedling establishment follows a distinct pattern which is partly controlled by hormones. Roots have high levels of cytokinins. By quantifying the fluctuations in endogenous cytokinins over time, further insight may be gained into the role of cytokinins during germination and seedling establishment. Radicles were excised from sterile Pisum sativum L. seeds after 30 min and 5 h imbibition. Seedlings germinated on agar were harvested after 1, 3, 6 and 9 days. The roots were divided into the root tip, root free zone, secondary root zone and from day 6, the secondary roots. Samples were purified by various chromatographic methods and endogenous cytokinins detected by LC(+)ES-MS. Benzyladenine levels doubled after 5 h imbibition and then gradually decreased over time. Low concentrations of cis-Zeatin (cZ) type cytokinins were detected in the radicle after 30 min imbibition. After 5 h imbibition, cis-zeatin riboside-5'-monophosphate had greatly increased. The total cytokinin content of th... (en)
Title
  • Spatial and temporal changes in endogenous cytokinins in developing pea roots
  • Spatial and temporal changes in endogenous cytokinins in developing pea roots (en)
  • Prostorové a časové změny u endogenních cytokininů ve vyvíjejících se kořenech hrachu (cs)
skos:prefLabel
  • Spatial and temporal changes in endogenous cytokinins in developing pea roots
  • Spatial and temporal changes in endogenous cytokinins in developing pea roots (en)
  • Prostorové a časové změny u endogenních cytokininů ve vyvíjejících se kořenech hrachu (cs)
skos:notation
  • RIV/61389030:_____/08:00322260!RIV09-AV0-61389030
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • S, Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika
  • 6
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 396268
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/08:00322260
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cytokinins; germination; Pisum (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [66368E92204E]
http://linked.open...i/riv/nazevZdroje
  • Planta
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 227
http://linked.open...iv/tvurceVysledku
  • Novák, Ondřej
  • Stirk, W. A.
  • Strnad, Miroslav
  • van Staden, J.
  • Tarkowski, Petr
  • Václavíková, Kateřina
http://linked.open...ain/vavai/riv/wos
  • 000254965100009
http://linked.open...n/vavai/riv/zamer
issn
  • 0032-0935
number of pages
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