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| rdf:type
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| Description
| - Byla vyvinuta jednoduchá imunoextrakční mikropurifikační metoda pro extrakci přirozeně se vyskytujících cytokininů kombinovaná s ultra-účinnou kapalinovou chromatografií spojenou s tandemovou hmotnostní spektrometrií. Nové extrakční a purifikační postupy byly optimalizovány na vzorcích topolového listí a analytická přesnost validována na 10-ti denních semenáčcích A. thaliana. Tento přístup může být použit pro rychlou, citlivou kvalitativní a/nebo kvantitativní analýzu víc než 50 přirozených cytokininů v mg množství rostlinných tkání. (cs)
- We have developed a simple, high-throughput batch immunoextraction (IAE) micropurification procedure for extracting a wide range of naturally occurring cytokinins (bases, ribosides, O- and N-glucosides, and nucleotides) from plant tissues in solutions that are compatible with ultra-performance liquid chromatography (UPLC), thereby facilitating sensitive subsequent analysis. The UPLC system was coupled to a tandem quadrupole mass spectrometer (MS/MS) equipped with an electrospray interface (ESI). Small (mg) amounts of tissues were purified by solid-phase extraction (SPE) followed by an immunoaffinity clean-up step and two fast chromatographic separations of most cytokinin metabolites (bases, ribosides, and 9-glucosides in the first, O-glucosides and nucleotides in the second). Using UPLC, the runs were up to 4-fold faster than in standard cytokinin analyses, and both retention times and injection volumes were less variable (RSDs, 0.15–0.3% and 1.0–5.5%, respectively). In multiple reaction monitoring...
- We have developed a simple, high-throughput batch immunoextraction (IAE) micropurification procedure for extracting a wide range of naturally occurring cytokinins (bases, ribosides, O- and N-glucosides, and nucleotides) from plant tissues in solutions that are compatible with ultra-performance liquid chromatography (UPLC), thereby facilitating sensitive subsequent analysis. The UPLC system was coupled to a tandem quadrupole mass spectrometer (MS/MS) equipped with an electrospray interface (ESI). Small (mg) amounts of tissues were purified by solid-phase extraction (SPE) followed by an immunoaffinity clean-up step and two fast chromatographic separations of most cytokinin metabolites (bases, ribosides, and 9-glucosides in the first, O-glucosides and nucleotides in the second). Using UPLC, the runs were up to 4-fold faster than in standard cytokinin analyses, and both retention times and injection volumes were less variable (RSDs, 0.15–0.3% and 1.0–5.5%, respectively). In multiple reaction monitoring... (en)
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| Title
| - Cytokinin profiling in plant tissues using ultra-performance liquid chromatography–electrospray tandem mass spectrometry
- Cytokininová analýza v rostlinných tkáních pomocí ultra-účinné kapalinové chromatografie-elektrosprej tandemové hmotnostní spektrometrie (cs)
- Cytokinin profiling in plant tissues using ultra-performance liquid chromatography–electrospray tandem mass spectrometry (en)
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| skos:prefLabel
| - Cytokinin profiling in plant tissues using ultra-performance liquid chromatography–electrospray tandem mass spectrometry
- Cytokininová analýza v rostlinných tkáních pomocí ultra-účinné kapalinové chromatografie-elektrosprej tandemové hmotnostní spektrometrie (cs)
- Cytokinin profiling in plant tissues using ultra-performance liquid chromatography–electrospray tandem mass spectrometry (en)
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| skos:notation
| - RIV/61389030:_____/08:00322259!RIV09-AV0-61389030
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(KAN200380801), S, Z(AV0Z50380511), Z(MSM6198959216)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/08:00322259
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Ultra-performance liquid chromatography (UPLC); Tandem mass spectrometry (MS/MS); Microextraction (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Doležal, Karel
- Novák, Ondřej
- Strnad, Miroslav
- Hauserová, Eva
- Amakorová, Petra
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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