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| rdf:type
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| Description
| - Byl studován a diskutován vliv transformace kvasinkou (Schizosaccharomyces pombe) cdc25 (Spcdc25) na tabáku (Nicotiana tabacum L.) a sledována podobnost buněčných parametrů jako při aplikaci cytokininů u netransformovaného typu. Také narůstaly hladiny škrobu a cukrů v porovnání s kontrolou. I když Spcdc25 transformace ukázala cytokininový účinek na studované charakteristicky, tak stanovení odhalilo výrazně nižší hladiny endogenních cytokininů v Spcdc25 transformantech. (cs)
- In plants, the G2/M control of cell cycle remains an elusive issue as doubts persist about activatory dephosphorylation—in other eukaryotes provided by CDC25 phosphatase and serving as a final all-or-nothing mitosis regulator. We report on the effects of tobacco (Nicotiana tabacum L., cv. Samsun) transformation with fission yeast (Schizosaccharomyces pombe) cdc25 (Spcdc25) on cell characteristics. Transformed cell suspension cultures showed higher dry mass accumulation during the exponential phase and clustered more circular cell phenotypes compared to chains of elongated WT cells. Similar cell parameters, as in the transformants, can be induced in WT by cytokinins. Spcdc25 cells, after cytokinin treatment, showed giant cell clusters and growth inhibition. In addition, Spcdc25 expression led to altered carbohydrate status: increased starch and soluble sugars with higher sucrose:hexoses ratio, inducible in WT by cytokinin treatment. Taken together, the Spcdc25 transformation had a cytokinin-like eff...
- In plants, the G2/M control of cell cycle remains an elusive issue as doubts persist about activatory dephosphorylation—in other eukaryotes provided by CDC25 phosphatase and serving as a final all-or-nothing mitosis regulator. We report on the effects of tobacco (Nicotiana tabacum L., cv. Samsun) transformation with fission yeast (Schizosaccharomyces pombe) cdc25 (Spcdc25) on cell characteristics. Transformed cell suspension cultures showed higher dry mass accumulation during the exponential phase and clustered more circular cell phenotypes compared to chains of elongated WT cells. Similar cell parameters, as in the transformants, can be induced in WT by cytokinins. Spcdc25 cells, after cytokinin treatment, showed giant cell clusters and growth inhibition. In addition, Spcdc25 expression led to altered carbohydrate status: increased starch and soluble sugars with higher sucrose:hexoses ratio, inducible in WT by cytokinin treatment. Taken together, the Spcdc25 transformation had a cytokinin-like eff... (en)
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| Title
| - Tobacco cells transformed with the fission yeast Spcdc25 mitotic inducer display growth and morphological characteristics as well as starch and sugar status evocable by cytokinin application
- Tabákové buňky transformované kvasnicovým Spcdc25 mitotickým induktorem ukazují růst a morfologické charakteristicky stejně jako stav škrobu a cukrů vyvolané po aplikaci cytokininů (cs)
- Tobacco cells transformed with the fission yeast Spcdc25 mitotic inducer display growth and morphological characteristics as well as starch and sugar status evocable by cytokinin application (en)
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| skos:prefLabel
| - Tobacco cells transformed with the fission yeast Spcdc25 mitotic inducer display growth and morphological characteristics as well as starch and sugar status evocable by cytokinin application
- Tabákové buňky transformované kvasnicovým Spcdc25 mitotickým induktorem ukazují růst a morfologické charakteristicky stejně jako stav škrobu a cukrů vyvolané po aplikaci cytokininů (cs)
- Tobacco cells transformed with the fission yeast Spcdc25 mitotic inducer display growth and morphological characteristics as well as starch and sugar status evocable by cytokinin application (en)
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| skos:notation
| - RIV/61389030:_____/08:00322257!RIV09-AV0-61389030
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - Z(AV0Z50380511), Z(MSM0021620858), Z(MSM6198959216)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/08:00322257
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Carbohydrate status; cdc25; Cell cycle (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - FR - Francouzská republika
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Plant Physiology and Biochemistry
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Novák, Ondřej
- Lipavská, H.
- Suchomelová-Mašková, P.
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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