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| Description
| - To study the effects of cytokinin O-glucosylation in monocots, maize (Zea mays L.) transformants harbouring the ZOG1 gene (encoding a zeatin O-glucosyltransferase from Phaseolus lunatus L.) under the control of the constitutive ubiquitin (Ubi) promoter were generated. The roots and leaves of the transformants had greatly increased levels of zeatin-O-glucoside. The vegetative characteristics of hemizygous and homozygous Ubi:ZOG1 plants resembled those of cytokinindeficient plants, including shorter stature, thinner stems, narrower leaves, smaller meristems, and increased root mass and branching. Transformant leaves had a higher chlorophyll content and increased levels of active cytokinins compared with those of non-transformed sibs. The Ubi:ZOG1 plants exhibited delayed senescence when grown in the spring/summer. While hemizygous transformants had reduced tassels with fewer spikelets and normal viable pollen, homozygotes had very small tassels and feminized tassel florets, resembl...
- To study the effects of cytokinin O-glucosylation in monocots, maize (Zea mays L.) transformants harbouring the ZOG1 gene (encoding a zeatin O-glucosyltransferase from Phaseolus lunatus L.) under the control of the constitutive ubiquitin (Ubi) promoter were generated. The roots and leaves of the transformants had greatly increased levels of zeatin-O-glucoside. The vegetative characteristics of hemizygous and homozygous Ubi:ZOG1 plants resembled those of cytokinindeficient plants, including shorter stature, thinner stems, narrower leaves, smaller meristems, and increased root mass and branching. Transformant leaves had a higher chlorophyll content and increased levels of active cytokinins compared with those of non-transformed sibs. The Ubi:ZOG1 plants exhibited delayed senescence when grown in the spring/summer. While hemizygous transformants had reduced tassels with fewer spikelets and normal viable pollen, homozygotes had very small tassels and feminized tassel florets, resembl... (en)
- Připravené transformanty kukuřice (Zea mays L.) nesoucí gen ZOG1 (kódující zeatin O-glukosyltransferázu z Phaseolus lunatus L.) pod konstitutivním ubiquitinovým promotorem (Ubi) byly využity pro studium vlivu O-glukosylace cytokininů v jednoděložných rostlinách. Kořeny a listy transformantů měly významně zvýšené hladiny zeatin O-glukosidu. Svým fenotypem se hemizygotní a homozygotní Ubi:ZOG1 rostliny podobaly rostlinám se sníženou hladinou cytokininů, včetně nižšího vzrůstu, tenčího stonku, menším meristémům, zvýšené hmotnosti a větvení kořenového systému. Listy transformantů měly vyšší hladinu chlorofylu a zvýšenou hladinu aktivních cytokininů ve srovnání s netransformovanými rostlinami. Ubi:ZOG1 rostliny vykazovaly zpomalenou senescenci během kultivace. Hemizygotní tranformanty měly redukované samčí květenství s normálním životaschopným pylem, homozygoty měly velmi malá samčí květenství, docházelo u nich k výrazné feminizaci. Tato modifikace reproduktivní fáze byla zcela neočekávaná a naznačuje f... (cs)
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| Title
| - Over-expression of a zeatin O-glucosylation gene in maize leads to growth retardation and tasselseed formation
- Over-expression of a zeatin O-glucosylation gene in maize leads to growth retardation and tasselseed formation (en)
- Zvýšená exprese genu pro zeatin O-glukosylaci v kukuřici vede ke zpomalení růstu a feminizaci květenství (cs)
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| skos:prefLabel
| - Over-expression of a zeatin O-glucosylation gene in maize leads to growth retardation and tasselseed formation
- Over-expression of a zeatin O-glucosylation gene in maize leads to growth retardation and tasselseed formation (en)
- Zvýšená exprese genu pro zeatin O-glukosylaci v kukuřici vede ke zpomalení růstu a feminizaci květenství (cs)
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| skos:notation
| - RIV/61389030:_____/08:00319079!RIV09-AV0-61389030
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(ME 868), Z(AV0Z50380511)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/08:00319079
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Corn; cytokinin; plant development; tasselseed (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Experimental Botany
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Malbeck, Jiří
- Vaňková, Radomíra
- Martin, R. C.
- Brugiere, N.
- Habben, J. E.
- Haines, S. C.
- Mok, D. W. S.
- Mok, M. C.
- Olson, J. M.
- Rodó, A. P.
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| http://linked.open...ain/vavai/riv/wos
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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