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  • Tobacco (Nicotiana tabacum L. var. xanthi) seedlings were treated with aqueous solutions of lead nitrate (Pb2+) at concentrations ranging from 0.4 mM to 2.4 mM for 24 h and from 25 μM to 200 μM for 7 days. The DNA damage measured by the comet assay was high in the root nuclei, but in the leaf nuclei a slight but significant increase in DNA damage could be demonstrated only after a 7-day treatment with 200 μM Pb2+. In tobacco plants growing for 6 weeks in soil polluted with Pb2+ severe toxic effects, expressed by the decrease in leaf area, and a slight but significant increase in DNA damage were observed. The tobacco plants with increased levels of DNA damage were severely injured and showed stunted growth, distorted leaves and brown root tips. The frequency of somatic mutations in tobacco plants growing in the Pb2+-polluted soil did not significantly increase. Analytical studies by inductively coupled plasma optical emission spectrometry demonstrate that after a 24-h treatment of tobacco with 2.4 m...
  • Tobacco (Nicotiana tabacum L. var. xanthi) seedlings were treated with aqueous solutions of lead nitrate (Pb2+) at concentrations ranging from 0.4 mM to 2.4 mM for 24 h and from 25 μM to 200 μM for 7 days. The DNA damage measured by the comet assay was high in the root nuclei, but in the leaf nuclei a slight but significant increase in DNA damage could be demonstrated only after a 7-day treatment with 200 μM Pb2+. In tobacco plants growing for 6 weeks in soil polluted with Pb2+ severe toxic effects, expressed by the decrease in leaf area, and a slight but significant increase in DNA damage were observed. The tobacco plants with increased levels of DNA damage were severely injured and showed stunted growth, distorted leaves and brown root tips. The frequency of somatic mutations in tobacco plants growing in the Pb2+-polluted soil did not significantly increase. Analytical studies by inductively coupled plasma optical emission spectrometry demonstrate that after a 24-h treatment of tobacco with 2.4 m... (en)
  • Semenáčky tabáku (Nicotiana tabacum var. xanthi) byly ovlivněny roztoky Pb (NO3)2 po dobu 24 h a 7 dní. Průkazné zvýšení poškození DNA, měřeno kometovým testem, bylo prokázáno pouze v kořenech semenáčku. Po kultivaci semenáčků tabáku v pudě kontaminované Pb (NO3)2 po dobu 6 týdnů bylo zvýšení poškození DNA vždy spojeno se silnou toxicitou (inhibice růstu semenáčků, nahnědlé a zdeformované listy). Zvýšení frekvence somatických mutací nebylo prokázáno. Zvýšení poškozené DNA je pravděpodobně podmíněné nekrotickou fragmentací DNA. (cs)
Title
  • Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants
  • Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants (en)
  • Hodnocení poškození DNA a mutagenity po aplikaci olova u rostlin tabaku (cs)
skos:prefLabel
  • Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants
  • Evaluation of DNA damage and mutagenicity induced by lead in tobacco plants (en)
  • Hodnocení poškození DNA a mutagenity po aplikaci olova u rostlin tabaku (cs)
skos:notation
  • RIV/61389030:_____/08:00307201!RIV08-AV0-61389030
http://linked.open.../vavai/riv/strany
  • 186;190
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA521/05/0500), V, Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 366780
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/08:00307201
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Comet assay; Nicotiana tabacum L. var. xanthi; Single-cell gel electrophoresis; Somatic mutations (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [7EC804BCC3D8]
http://linked.open...i/riv/nazevZdroje
  • Mutation Research - Genetic Toxicology and Environmental Mutagenesis
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 652
http://linked.open...iv/tvurceVysledku
  • Száková, J.
  • Gichner, Tomáš
  • Žnidar, I.
http://linked.open...n/vavai/riv/zamer
issn
  • 1383-5718
number of pages
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