About: DNA damage in potato plants induced by cadmium, ethyl methanesulphonate and gama-rays     Goto   Sponge   NotDistinct   Permalink

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  • Zakořeněné řízky brambor Solanum tuberosum kultivar Korela byly ovlivněny těžkým kovem kadmiem. Poškození DNA měřeno technikou komet, bylo prokázáno jen v kořenech rostlin brambor, zatímco v listech ke zvýšení poškození DNA nedošlo. Analýza obsahu kadmia za použití atomového absorpčního spektrometru prokázalo, že kořený akumulují až 9x více kadmia než listy. Po aplikaci ethyl methansulfonátu a γ- paprsků došlo k poškození DNA jak v kořenech tak v listech. (cs)
  • We have calibrated the alkaline protocol of the plant comet (Single Cell Gel Electrophoresis) assay as a method for detecting the extent of induced DNA damage in potato plants (Solanum tuberosum L. cultivar Korela). After 2 and 24 h treatments of the rooted cuttings with the heavy metal cadmium (Cd2+), a dose–response increase in DNA damage was noted versus controls in root nuclei. With a 24 h recovery period, the Cd2+-induced DNA damage in roots increased significantly. No significant increase in DNA damage was demonstrated in leaf nuclei after 24 h Cd2+ treatments, but continuous Cd2+ treatments for 2 weeks resulted in an increase in leaf DNA damage. This increase may be however associated with necrotic and apoptotic DNA fragmentation, as the affected plants had inhibited growth and distorted yellowish leaves. For comparison, the monofunctional alkylating agent ethyl methanesulphonate, and γ-rays were assessed for induced DNA damage. Analysis of the accumulation of cadmium by inductively coupled ...
  • We have calibrated the alkaline protocol of the plant comet (Single Cell Gel Electrophoresis) assay as a method for detecting the extent of induced DNA damage in potato plants (Solanum tuberosum L. cultivar Korela). After 2 and 24 h treatments of the rooted cuttings with the heavy metal cadmium (Cd2+), a dose–response increase in DNA damage was noted versus controls in root nuclei. With a 24 h recovery period, the Cd2+-induced DNA damage in roots increased significantly. No significant increase in DNA damage was demonstrated in leaf nuclei after 24 h Cd2+ treatments, but continuous Cd2+ treatments for 2 weeks resulted in an increase in leaf DNA damage. This increase may be however associated with necrotic and apoptotic DNA fragmentation, as the affected plants had inhibited growth and distorted yellowish leaves. For comparison, the monofunctional alkylating agent ethyl methanesulphonate, and γ-rays were assessed for induced DNA damage. Analysis of the accumulation of cadmium by inductively coupled ... (en)
Title
  • DNA damage in potato plants induced by cadmium, ethyl methanesulphonate and gama-rays
  • DNA damage in potato plants induced by cadmium, ethyl methanesulphonate and gama-rays (en)
  • Poškození DNA u rostlin brambor po aplikaci kadmia, ethyl methansulfonátu a γ- paprsků (cs)
skos:prefLabel
  • DNA damage in potato plants induced by cadmium, ethyl methanesulphonate and gama-rays
  • DNA damage in potato plants induced by cadmium, ethyl methanesulphonate and gama-rays (en)
  • Poškození DNA u rostlin brambor po aplikaci kadmia, ethyl methansulfonátu a γ- paprsků (cs)
skos:notation
  • RIV/61389030:_____/08:00099655!RIV08-AV0-61389030
http://linked.open.../vavai/riv/strany
  • 113;119
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA521/05/0500), V, Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 364036
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/08:00099655
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Comet assay; Solanum tuberosum L. (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [9A96E4A5C81B]
http://linked.open...i/riv/nazevZdroje
  • Environmental and Experimental Botany
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 62
http://linked.open...iv/tvurceVysledku
  • Száková, J.
  • Mukherjee, A.
  • Gichner, Tomáš
  • Patková, Zdeňka
  • Žnidar, I.
http://linked.open...n/vavai/riv/zamer
issn
  • 0098-8472
number of pages
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