About: Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography–mass spectrometry Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Polyclonal antibodies with high specificity for C1-immobilised (+)-cis,trans-abscisic acid (ABA) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of an inummoaffinity chromatography (IAQ gel. The detection limit of the ELISA was approximately 4.6 x 10(-10) mol/L. Sensitive electrospray liquid chromatography-mass; spectrometry (LC-ESI-MS) methods, were also developed with detection limits below 0.1 x 10(-12) mol. The IAC allowed quick, single-step processing of samples prior to the analyses. The LC-ESI-MS and LC-ELISA techniques were used for comparative estimation of endogenous ABA levels in immumoaffinity purified extracts of normal and water-stressed Nicotiana tabacum L. leaves. The analytical approaches were validated using deuterium- and tritium-labelled internal standards, respectively. The LAC method was found to be highly effective, sensitive and convenient for isolating the target analyte from plant material. Polyclonal antibodies with high specificity for C1-immobilised (+)-cis,trans-abscisic acid (ABA) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of an inummoaffinity chromatography (IAQ gel. The detection limit of the ELISA was approximately 4.6 x 10(-10) mol/L. Sensitive electrospray liquid chromatography-mass; spectrometry (LC-ESI-MS) methods, were also developed with detection limits below 0.1 x 10(-12) mol. The IAC allowed quick, single-step processing of samples prior to the analyses. The LC-ESI-MS and LC-ELISA techniques were used for comparative estimation of endogenous ABA levels in immumoaffinity purified extracts of normal and water-stressed Nicotiana tabacum L. leaves. The analytical approaches were validated using deuterium- and tritium-labelled internal standards, respectively. The LAC method was found to be highly effective, sensitive and convenient for isolating the target analyte from plant material. (en) Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography–mass spectrometry. (cs)
Title	Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography–mass spectrometry Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography–mass spectrometry (en) Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography–mass spectrometry (cs)
skos:prefLabel	Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography–mass spectrometry Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography–mass spectrometry (en) Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography–mass spectrometry (cs)
skos:notation	RIV/61389030:_____/07:00305972!RIV08-AV0-61389030
http://linked.open.../vavai/riv/strany	162;173
http://linked.open...avai/riv/aktivita	P V Z
http://linked.open...avai/riv/aktivity	P(IBS5038351), P(LC06034), V, Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2008
http://linked.open...aciTvurceVysledku	Havlíček, Libor Novák, Ondřej Strnad, Miroslav Turečková, Veronika
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	425521
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/07:00305972
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	abscisic acid; immunoaffinity chromatography; liquid chromatography-mass spectrometry (en)
http://linked.open.../riv/klicoveSlovo	liquid chromatography-mass spectrometry abscisic acid immunoaffinity chromatography
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[4A4DC24DE913]
http://linked.open...i/riv/nazevZdroje	Journal of Chromatography B-Analytical Technologies in the Biomedical and Life Sciences
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Regulation of morphogenesis of plant cells and organs Immunodiagnostics of natural hormonal substances
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	847
http://linked.open...iv/tvurceVysledku	Havlíček, Libor Novák, Ondřej Strnad, Miroslav Hradecká, Veronika
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Modulation of signalling and regulatory pathways in normal and cancer cells
issn	1570-0232
number of pages	12 (xsd:int)

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software