About: Chitinase isozymes induced by TYMV and Leptosphaeria maculans during compatible and incompatible interaction with Brassica napus     Goto   Sponge   NotDistinct   Permalink

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Description
  • V listech Brassica napus infikovaných virem žluté mozaiky vodnice a houbovým patogenem Leptosphaeria maculans se akumulují extracelulární chitinasy při inkompatibilní i kompatibilní interakci. Proteiny apoplastu byly analyzovány pomocí PAGE za nativních podmínek a v gelu detekovány kyselé i bazické isoenzymy chitinas. (cs)
  • Accumulation of extracellular chitinases in Brassica napus plants infected with Turnip yellow mosaic virus (TYMV) and fungal pathogen Leptosphaeria maculans was studied in both compatible and incompatible interaction. Analysis of apoplast fluid by means of non-denaturing anodic and cathodic PAGE followed by in-gel detection of chitinase activity revealed a number of chitinase isozymes. TYMV induced 8 acidic and 4 basic isozymes in a systemic way. Except for one acidic and one basic isozyme, all other chitinases were also constitutively present in low amounts in mock inoculated control. In TYMV systemically infected plants, chitinases were detected in leaves expressing symptoms as well as in symptomless ones. Both virulent and avirulent L. maculans isolates induced production of chitinase isozymes in cotyledons in a time dependent manner. Some of them were present in plants constitutively and their content increased after inoculation. Three of five acidic and two of three basic isozymes responded ...
  • Accumulation of extracellular chitinases in Brassica napus plants infected with Turnip yellow mosaic virus (TYMV) and fungal pathogen Leptosphaeria maculans was studied in both compatible and incompatible interaction. Analysis of apoplast fluid by means of non-denaturing anodic and cathodic PAGE followed by in-gel detection of chitinase activity revealed a number of chitinase isozymes. TYMV induced 8 acidic and 4 basic isozymes in a systemic way. Except for one acidic and one basic isozyme, all other chitinases were also constitutively present in low amounts in mock inoculated control. In TYMV systemically infected plants, chitinases were detected in leaves expressing symptoms as well as in symptomless ones. Both virulent and avirulent L. maculans isolates induced production of chitinase isozymes in cotyledons in a time dependent manner. Some of them were present in plants constitutively and their content increased after inoculation. Three of five acidic and two of three basic isozymes responded ... (en)
Title
  • Chitinase isozymes induced by TYMV and Leptosphaeria maculans during compatible and incompatible interaction with Brassica napus
  • Isoenzymy chitinas indukované TYMV a Leptosphaeria maculans při kompatibilní a inkompatibilní interakci u Brassica napus (cs)
  • Chitinase isozymes induced by TYMV and Leptosphaeria maculans during compatible and incompatible interaction with Brassica napus (en)
skos:prefLabel
  • Chitinase isozymes induced by TYMV and Leptosphaeria maculans during compatible and incompatible interaction with Brassica napus
  • Isoenzymy chitinas indukované TYMV a Leptosphaeria maculans při kompatibilní a inkompatibilní interakci u Brassica napus (cs)
  • Chitinase isozymes induced by TYMV and Leptosphaeria maculans during compatible and incompatible interaction with Brassica napus (en)
skos:notation
  • RIV/61389030:_____/07:00099891!RIV08-AV0-61389030
http://linked.open.../vavai/riv/strany
  • 507;513
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/03/0353), V, Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 413547
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/07:00099891
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Chitinase; TYMV; Leptosphaeria maculans; Brassica napus; PR-proteins (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [E420D4277988]
http://linked.open...i/riv/nazevZdroje
  • Biologia Plantarum
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 51
http://linked.open...iv/tvurceVysledku
  • Burketová, Lenka
  • Valentová, O.
  • Profotová, B.
http://linked.open...n/vavai/riv/zamer
issn
  • 0006-3134
number of pages
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