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| Description
| - Ultra-performance liquid chromatography-tandem mass spectrometry was used to establish the cytokinin profile of the bryophyte Physcomitrella patens (Hedw.) B. S. G.; of 40 analyzed cytokinins, 20 were detected. cis-Zeatin-riboside-O-glucoside, N-6-(Delta(2)-isopentenyl) adenosine-5'-monophosphate (iPRMP), and trans-zeatin-riboside-O-glucoside were the most abundant intracellular cytokinins. In addition, the aromatic cytokinins N 6-benzyladenosine (BAR), N 6-benzyladenine, meta-, and orthotopolin were detected. Unexpectedly, the most abundant extracellular cytokinin was the nucleotide iPRMP, and its identity was confirmed by quadrupole time-of-flight mass spectrometry. The effects of overexpressing a heterologous cytokinin oxidase/ dehydrogenase (CKX; EC 1.4.3.18/ 1.5.99.12) gene (AtCKX2 from Arabidopsis [ Arabidopsis thaliana]) on the intracellular and extracellular distribution of cytokinins was assessed. In cultures of CKX-transformed plants, ultra-performance liquid chromatography-tandem mass sp...
- Ultra-performance liquid chromatography-tandem mass spectrometry was used to establish the cytokinin profile of the bryophyte Physcomitrella patens (Hedw.) B. S. G.; of 40 analyzed cytokinins, 20 were detected. cis-Zeatin-riboside-O-glucoside, N-6-(Delta(2)-isopentenyl) adenosine-5'-monophosphate (iPRMP), and trans-zeatin-riboside-O-glucoside were the most abundant intracellular cytokinins. In addition, the aromatic cytokinins N 6-benzyladenosine (BAR), N 6-benzyladenine, meta-, and orthotopolin were detected. Unexpectedly, the most abundant extracellular cytokinin was the nucleotide iPRMP, and its identity was confirmed by quadrupole time-of-flight mass spectrometry. The effects of overexpressing a heterologous cytokinin oxidase/ dehydrogenase (CKX; EC 1.4.3.18/ 1.5.99.12) gene (AtCKX2 from Arabidopsis [ Arabidopsis thaliana]) on the intracellular and extracellular distribution of cytokinins was assessed. In cultures of CKX-transformed plants, ultra-performance liquid chromatography-tandem mass sp... (en)
- Metodou UPLC-MS/MS byl stanoven profil cytokininů v mechu Physcomitrella patens (Hedw.) B.S.G.; z celkového počtu 40 analyzovaných cytokininů bylo detekováno 20 derivátů. Cis-zeatinribosid-O-glukosid, N6-(Δ2-isopentenyl)adenosin-5´-monofosfát (iPRMP) a trans-zeatinribosid-O-glukosid byly nejhojněji se vyskytujícími intracelulárními cytokininy; kromě nich byla v buňkách mechu zjištěna také přítomnost aromatických cytokininů N6-benzyladenosinu (BAR), N6-benzyladeninu a meta- i orto-topolinů. Nejvíce zastoupeným extracelulárním cytokininem byl nukleotid iPRMP, jehož identita byla potvrzena na základě Q-Tof hmotové spektrometrie. V práci byly sledovány účinky exprese genu AtCKX2 z Arabidopsis thaliana kódujícího cytokininoxidázu/dehydrogenázu (CKX, EC 1.4.3.18/1.5.99.12) na vnitrobuněčnou i mimobuněčnou lokalizaci cytokininů. V kulturách CKX-transformovaných rostlin byl metodou UPLC-MS/MS zjištěn značný pokles extracelulárních koncentrací N6-(Δ2-isopentenyl)adeninu (iP) a N6-(Δ2-isopentenyl)adenosinu (... (cs)
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| Title
| - Cytokinins in the bryophyte Physcomitrella patens: Analyses of activity, distribution, and cytokinin oxidase/dehydrogenase overexpression reveal the role of extracellular cytokinins
- Cytokinins in the bryophyte Physcomitrella patens: Analyses of activity, distribution, and cytokinin oxidase/dehydrogenase overexpression reveal the role of extracellular cytokinins (en)
- Cytokininy v mechu Physcomitrella patens: Stanovení aktivity, distribuce a exprese cytokininoxidázy/dehydrogenázy indikují důležitost extracelulárních cytokininů (cs)
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| skos:prefLabel
| - Cytokinins in the bryophyte Physcomitrella patens: Analyses of activity, distribution, and cytokinin oxidase/dehydrogenase overexpression reveal the role of extracellular cytokinins
- Cytokinins in the bryophyte Physcomitrella patens: Analyses of activity, distribution, and cytokinin oxidase/dehydrogenase overexpression reveal the role of extracellular cytokinins (en)
- Cytokininy v mechu Physcomitrella patens: Stanovení aktivity, distribuce a exprese cytokininoxidázy/dehydrogenázy indikují důležitost extracelulárních cytokininů (cs)
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| skos:notation
| - RIV/61389030:_____/07:00099665!RIV08-AV0-61389030
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA522/06/0703), P(IAA600380701), V, Z(AV0Z50380511), Z(MSM6198959216)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/07:00099665
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - ARABIDOPSIS-THALIANA; OXIDASE ACTIVITY; ATP/ADP ISOPENTENYLTRANSFERASES (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Dobrev, Petre
- Motyka, Václav
- Novák, Ondřej
- Strnad, Miroslav
- von Schwartzenberg, K.
- Blaschke, H.
- Nunez, M. F.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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