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  • Engineering transgenic plants with reduced cytokinin (Ck) contents is a way to analyze the role of these hormones in growth and development. Cytokinin oxidase/dehydrogenase (CKO) genes are good candidates to promote Ck deficiency. They code for enzymes degrading Cks and generally belong to multigene families. Plants constitutively expressing naturally occurring CKO genes that code for secreted or vacuolar enzymes have been described. We report on Arabidopsis transgenics constitutively overexpressing the secreted native form or an engineered non-secreted form of the maize CKO1 enzyme. Severity of phenotype symptoms (increased root system, reduced size of aerial parts and defects in seed development) was clearly correlated with the level of enzyme activity. Aerial part was especially affected in plants overexpressing the non-secreted enzyme, even at low activity level. In all strong overexpressers, zeatin-type metabolites were highly depleted compared to isopentenyladenine-type metabolites. AtIPT gen...
  • Engineering transgenic plants with reduced cytokinin (Ck) contents is a way to analyze the role of these hormones in growth and development. Cytokinin oxidase/dehydrogenase (CKO) genes are good candidates to promote Ck deficiency. They code for enzymes degrading Cks and generally belong to multigene families. Plants constitutively expressing naturally occurring CKO genes that code for secreted or vacuolar enzymes have been described. We report on Arabidopsis transgenics constitutively overexpressing the secreted native form or an engineered non-secreted form of the maize CKO1 enzyme. Severity of phenotype symptoms (increased root system, reduced size of aerial parts and defects in seed development) was clearly correlated with the level of enzyme activity. Aerial part was especially affected in plants overexpressing the non-secreted enzyme, even at low activity level. In all strong overexpressers, zeatin-type metabolites were highly depleted compared to isopentenyladenine-type metabolites. AtIPT gen... (en)
  • Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene. (cs)
Title
  • Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene
  • Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene (en)
  • Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene (cs)
skos:prefLabel
  • Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene
  • Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene (en)
  • Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene (cs)
skos:notation
  • RIV/61389030:_____/06:00083205!RIV07-AV0-61389030
http://linked.open.../vavai/riv/strany
  • 114;122
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 494877
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/06:00083205
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Arabidopsis thaliana; Cytokinin oxidase/dehydrogenase; Homeostasis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • IE - Irsko
http://linked.open...ontrolniKodProRIV
  • [4BE347D7E986]
http://linked.open...i/riv/nazevZdroje
  • Plant Science
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 171
http://linked.open...iv/tvurceVysledku
  • Tarkowski, Petr
  • Nogué, F.
  • Sandberg, G.
  • Kopečný, D.
  • Laloue, M.
  • Houba-Hérin, N.
  • Bouchez-Mahiout, I.
  • Laurière, M.
  • Majira, M.
http://linked.open...n/vavai/riv/zamer
issn
  • 0168-9452
number of pages
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