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Description
| - Fosfolipidový signální systém je důležitou součástí signálních přenosů u eukaryot. U rostlin hraje klíčovou roli nejen v růstu a vývoji, ale také při odezvě rostlin na stres včetně napadení patogeny. Sledována byla účast fosfolipasy C (PLC, EC 3.1.4.11) a fosfolipasy D (PLD, EC 3.1.4.4) při časné odezvě Brassica napus na chemické induktory systémově získané resistence (SAR): kyselinu salicylovou (SA), benzothiadiazol (BTH) a na induktor indukované systémové resistence (ISR), metyljasmonát (MeJA). Byla zjištěna rychlá aktivace in vitro aktivity PLC a tří enzymaticky rozdílných forem PLD: PLD alfa, PLD beta/gama (PLD závislá na fofatidylinositol- 4,5- bisfosfátu (PIP2)) a oleátem stimulované PLD delta. Nejsilnější odezva byla zjištěna v případě cytosolické PLD beta/gama po ošetření BTH. PLD delta 7 byla identifikována v listech B. napus a byla velmi rychle aktivována ošetřením MeJA. Nárůst obsahu proteinu byl zjištěn pouze u PLD beta/gama a delta po indukci ISR, a to později než byla zaznamenána jej... (cs)
- Phospholipid signaling is an important component in eukaryotic signal transduction pathways. In plants, it plays a key role in growth and development as well as in responses to environmental stresses, including pathogen attack. We investigated the involvement of both phospholipase C (PLC, EC 3.1.4.11) and D (PLD, EC 3.1.4.4) in early responses to the treatment of Brassica napus plants with the chemical inducers of systemic acquired resistance (SAR): salicylic acid (SA), benzothiadiazole (BTH), and with the inducer mediating the induced systemic resistance (ISR) pathway, methyl jasmonate (MeJA). Rapid activation (within 0.5–6 h treatment) of the in vitro activity level was found for phosphatidyl inositol 4,5 bisphosphate (PIP2)-specific PLC (PI-PLC) and three enzymatically different forms of PLD: conventional PLDα, PIP2-dependent PLD β/γ, and oleate-stimulated PLDδ. The strongest response was found in case of cytosolic PIP2-dependent PLD β/γ after BTH treatment. PLDδ was identified in B. napu...
- Phospholipid signaling is an important component in eukaryotic signal transduction pathways. In plants, it plays a key role in growth and development as well as in responses to environmental stresses, including pathogen attack. We investigated the involvement of both phospholipase C (PLC, EC 3.1.4.11) and D (PLD, EC 3.1.4.4) in early responses to the treatment of Brassica napus plants with the chemical inducers of systemic acquired resistance (SAR): salicylic acid (SA), benzothiadiazole (BTH), and with the inducer mediating the induced systemic resistance (ISR) pathway, methyl jasmonate (MeJA). Rapid activation (within 0.5–6 h treatment) of the in vitro activity level was found for phosphatidyl inositol 4,5 bisphosphate (PIP2)-specific PLC (PI-PLC) and three enzymatically different forms of PLD: conventional PLDα, PIP2-dependent PLD β/γ, and oleate-stimulated PLDδ. The strongest response was found in case of cytosolic PIP2-dependent PLD β/γ after BTH treatment. PLDδ was identified in B. napu... (en)
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Title
| - Involvement of phospholipases C and D in early response to SAR and ISR inducers in Brassica napus plants
- Involvement of phospholipases C and D in early response to SAR and ISR inducers in Brassica napus plants (en)
- Zapojení fosfolipáz C a D v časné odezvě induktorů SAR a ISR u řepky olejky (cs)
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skos:prefLabel
| - Involvement of phospholipases C and D in early response to SAR and ISR inducers in Brassica napus plants
- Involvement of phospholipases C and D in early response to SAR and ISR inducers in Brassica napus plants (en)
- Zapojení fosfolipáz C a D v časné odezvě induktorů SAR a ISR u řepky olejky (cs)
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skos:notation
| - RIV/61389030:_____/06:00080199!RIV07-AV0-61389030
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(GA522/03/0353), Z(AV0Z50380511)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/06:00080199
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Brassica napus; Induced resistance; Phospholipase C and D (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - FR - Francouzská republika
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Plant Physiology and Biochemistry
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Martinec, Jan
- Novotná, Z.
- Burketová, Lenka
- Valentová, O.
- Profotová, B.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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