About: DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We applied the alkaline version of the single-cell gel electrophoresis (comet) assay to roots and leaves of tobacco (Nicotiana tabacum var. xanthi) seedlings or isolated leaf nuclei treated with: (1) the alkylating agent ethyl methanesulphonate, (2) necrotic heat treatments at 50 °C, and (3) DNase-I. All three treatments induced a dose-dependent increase in DNA migration, expressed as percentage of tail DNA. A comparison of the fluorochrome DNA dyes ethidium bromide, DAPI and YOYO-1 demonstrated that for the alkaline version of the comet assay in plants, the commonly used fluorescent dye ethidium bromide can be used with the same efficiency as DAPI or YOYO-1. We applied the alkaline version of the single-cell gel electrophoresis (comet) assay to roots and leaves of tobacco (Nicotiana tabacum var. xanthi) seedlings or isolated leaf nuclei treated with: (1) the alkylating agent ethyl methanesulphonate, (2) necrotic heat treatments at 50 °C, and (3) DNase-I. All three treatments induced a dose-dependent increase in DNA migration, expressed as percentage of tail DNA. A comparison of the fluorochrome DNA dyes ethidium bromide, DAPI and YOYO-1 demonstrated that for the alkaline version of the comet assay in plants, the commonly used fluorescent dye ethidium bromide can be used with the same efficiency as DAPI or YOYO-1. (en) Nebyly prokázány dozdily v kometovém testu při barvení DNA pomoci 3 fluorochromů. Po aplikaci EMS, vysoké teploty a DNAsy-I bylo po alkalické elektroforéze zjištěno zvýšení poškození DNA v jádrech listů a kořenů heterozygotního tabáku Nicotiana tabacum var. xanthi. Výsledky dokazují, že kometový test je schopen detekovat jak genotoxické tak nekrotické poškození DNA. (cs)
Title	DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I Barvení fluorochromy EtBr, DAPI, a YOYO v kometovém testu u tabáku po aplikaci EMS, vysoké teploty a DNase -I (cs) DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I (en)
skos:prefLabel	DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I Barvení fluorochromy EtBr, DAPI, a YOYO v kometovém testu u tabáku po aplikaci EMS, vysoké teploty a DNase -I (cs) DNA staining with the fluorochromes EtBr, DAPI and YOYO-1 in the comet assay with tobacco plants after treatment with ethyl methanesulphonate, hyperthermia and DNase-I (en)
skos:notation	RIV/61389030:_____/06:00050775!RIV07-AV0-61389030
http://linked.open.../vavai/riv/strany	17;21
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA521/05/0500), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	1-2
http://linked.open...vai/riv/dodaniDat	2007
http://linked.open...aciTvurceVysledku	Velemínský, Jiří Gichner, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	472120
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/06:00050775
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA migration; Nicotiana tabacum; Single-cell gel electrophoresis (en)
http://linked.open.../riv/klicoveSlovo	Nicotiana tabacum Single-cell gel electrophoresis DNA migration
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[A1B268A12CF9]
http://linked.open...i/riv/nazevZdroje	Mutation Research - Genetic Toxicology and Environmental Mutagenesis
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Induced DNA damage, mutagenicity, homologous recombination and apoptosis as endpoints for monitoring effects of pollutants in tobacco and potato
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	605
http://linked.open...iv/tvurceVysledku	Mukherjee, A. Gichner, Tomáš Velemínský, Jiří
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	1383-5718
number of pages	5 (xsd:int)

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software