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  • Identification of stress-induced mitochondrial proteins in cultured tobacco cells. (cs)
  • Under stress conditions, small and large heat-shock proteins cooperatively fulfil molecular chaperone activities within many cellular compartments, including mitochondria. Here, we report the identification and characterization of 10 newly synthesized low-molecular-weight heat stress-induced proteins (SIPs) found solely in the mitochondrial fraction of suspension-cultured cells of tobacco (Nicotiana tabacum L., strain Virginia Bright Italia-0). These SIPs were shown to be encoded by the nuclear genome and none was detected in non-stressed cells. Mass spectroscopy analysis revealed that eight SIPs belonged to the group of small heat-shock proteins, whilst one protein shared significant homology with transcription factors. De novo synthesis of all SIPs was detected within 10 min of the commencement of heat stress and continued for the whole 12 h stress interval. Moreover, nine of the SIPs were stable for 24 h following stress termination. SIP1, which showed the most intense synthesis profile, was fou...
  • Under stress conditions, small and large heat-shock proteins cooperatively fulfil molecular chaperone activities within many cellular compartments, including mitochondria. Here, we report the identification and characterization of 10 newly synthesized low-molecular-weight heat stress-induced proteins (SIPs) found solely in the mitochondrial fraction of suspension-cultured cells of tobacco (Nicotiana tabacum L., strain Virginia Bright Italia-0). These SIPs were shown to be encoded by the nuclear genome and none was detected in non-stressed cells. Mass spectroscopy analysis revealed that eight SIPs belonged to the group of small heat-shock proteins, whilst one protein shared significant homology with transcription factors. De novo synthesis of all SIPs was detected within 10 min of the commencement of heat stress and continued for the whole 12 h stress interval. Moreover, nine of the SIPs were stable for 24 h following stress termination. SIP1, which showed the most intense synthesis profile, was fou... (en)
Title
  • Identification of stress-induced mitochondrial proteins in cultured tobacco cells
  • Identification of stress-induced mitochondrial proteins in cultured tobacco cells (en)
  • Identification of stress-induced mitochondrial proteins in cultured tobacco cells (cs)
skos:prefLabel
  • Identification of stress-induced mitochondrial proteins in cultured tobacco cells
  • Identification of stress-induced mitochondrial proteins in cultured tobacco cells (en)
  • Identification of stress-induced mitochondrial proteins in cultured tobacco cells (cs)
skos:notation
  • RIV/61389030:_____/05:00325870!RIV09-AV0-61389030
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(KSK5052113), Z(AV0Z50380511), Z(MSM 113100003)
http://linked.open...iv/cisloPeriodika
  • 1
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http://linked.open...aciTvurceVysledku
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  • 524135
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/05:00325870
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • HEAT-SHOCK-PROTEIN; ARABIDOPSIS-THALIANA L; POLYACRYLAMIDE-GELS (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DK - Dánské království
http://linked.open...ontrolniKodProRIV
  • [E1E940D2C092]
http://linked.open...i/riv/nazevZdroje
  • Physiologia Plantarum
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 124
http://linked.open...iv/tvurceVysledku
  • Hájek, Tomáš
  • Honys, David
  • Čapková, Věra
  • Witters, E.
http://linked.open...ain/vavai/riv/wos
  • 000228975000003
http://linked.open...n/vavai/riv/zamer
issn
  • 0031-9317
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