About: Modification of Cytokinin Levels in Potato via Expression of the Petunia hybrida Sho Gene     Goto   Sponge   NotDistinct   Permalink

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Description
  • Gen Sho z Petunie kóduje enzym biosyntézy cytokininů. Zde uvádíme vliv exprese genu Sho na vývoj bramboru a srovnání s rostlinami bramboru, do nichž byl vnesen gen ipt z Agrobacterium. Středně silná exprese genu Sho umožnila vývoj dostatečného kořenového systému, aby rostliny mohly být kultivovány v půdě. Nejnápadnějším vlivem transformace genem Sho byla nadměrná tvorba stonků , pozdější tvorba hlíz a jejich menší velikost a kratší doba dormance. Exprese genu Sho vede silnému zvýšení obsahu 2iP glukosidů, velmi vysoká exprese i k nárůstu obsahu glukosidů zeatinu. Data ukazují, že pomocí exprese genu Sho lze získat životaschopné rostliny s vysokým obsahem cytokininů. (cs)
  • The Sho gene from Petunia hybrida encodes an enzyme for cytokinin synthesis. Here we report on the effects of Shogene expression on potato development. In contrast to transgenic potato expressing the Agrobacterium ipt gene, moderate Sho expression resulted in sufficient root development that allowed the cultivation of the Sho transformants in soil. The most pronounced effects detectable in these lines were an enhanced shoot production, delayed tuber formation, significant reduction in tuber size, and inhibition of tuber dormancy. Sho expression predominantly associated with a strong increase in 2iP glucosides, accompanied by an increase in zeatin glucosides in lines with very high Sho expression levels. The data demonstrate that it is possible to produce viable plants with enhanced cytokinin levels via constitutive Sho expression, which allows an assessment of cytokinin effects in all organs.
  • The Sho gene from Petunia hybrida encodes an enzyme for cytokinin synthesis. Here we report on the effects of Shogene expression on potato development. In contrast to transgenic potato expressing the Agrobacterium ipt gene, moderate Sho expression resulted in sufficient root development that allowed the cultivation of the Sho transformants in soil. The most pronounced effects detectable in these lines were an enhanced shoot production, delayed tuber formation, significant reduction in tuber size, and inhibition of tuber dormancy. Sho expression predominantly associated with a strong increase in 2iP glucosides, accompanied by an increase in zeatin glucosides in lines with very high Sho expression levels. The data demonstrate that it is possible to produce viable plants with enhanced cytokinin levels via constitutive Sho expression, which allows an assessment of cytokinin effects in all organs. (en)
Title
  • Modification of Cytokinin Levels in Potato via Expression of the Petunia hybrida Sho Gene
  • Modifikace hladiny cytokininů u bramboru expresí genu Sho z Petunia hybrida (cs)
  • Modification of Cytokinin Levels in Potato via Expression of the Petunia hybrida Sho Gene (en)
skos:prefLabel
  • Modification of Cytokinin Levels in Potato via Expression of the Petunia hybrida Sho Gene
  • Modifikace hladiny cytokininů u bramboru expresí genu Sho z Petunia hybrida (cs)
  • Modification of Cytokinin Levels in Potato via Expression of the Petunia hybrida Sho Gene (en)
skos:notation
  • RIV/61389030:_____/05:00030613!RIV06-AV0-61389030
http://linked.open.../vavai/riv/strany
  • 615;618
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika
  • 5
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 530903
http://linked.open...ai/riv/idVysledku
  • RIV/61389030:_____/05:00030613
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • cytokinins; ipt; petunia (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [9AD592879391]
http://linked.open...i/riv/nazevZdroje
  • Transgenic Research
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 14
http://linked.open...iv/tvurceVysledku
  • Macháčková, Ivana
  • Malbeck, Jiří
  • Meyer, P.
  • Zubko, E.
http://linked.open...n/vavai/riv/zamer
issn
  • 0962-8819
number of pages
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