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Description
| - To prevent leaf senescence of young transplants or excised shoots during storage under dark and cold conditions, the cytokinin biosynthetic gene isopentenyl transferase (ipt) was placed under the control of a cold-inducible promoter cor15a from Arabidopsis thaliana and introduced into Petuniaxhybrida ‘Marco Polo Odyssey’ and Dendranthemaxgrandiflorum (chrysanthemum) ‘Iridon’. Transgenic cor15a-ipt petunia and chrysanthemum plants and excised leaves remained green and healthy during prolonged dark storage (4 weeks at 25 °C) after an initial exposure to a brief cold-induction period (4 °C for 72 h). However, cor15a-ipt chrysanthemum plants and excised leaves that were not exposed to a cold-induction period, senesced under the same dark storage conditions. Regardless of cold-induction treatment, leaves and plants of non-transformed plants senesced under prolonged dark storage. Analysis of ipt expression indicated a marked increase in gene expression in intact transgenic plants as well ...
- To prevent leaf senescence of young transplants or excised shoots during storage under dark and cold conditions, the cytokinin biosynthetic gene isopentenyl transferase (ipt) was placed under the control of a cold-inducible promoter cor15a from Arabidopsis thaliana and introduced into Petuniaxhybrida ‘Marco Polo Odyssey’ and Dendranthemaxgrandiflorum (chrysanthemum) ‘Iridon’. Transgenic cor15a-ipt petunia and chrysanthemum plants and excised leaves remained green and healthy during prolonged dark storage (4 weeks at 25 °C) after an initial exposure to a brief cold-induction period (4 °C for 72 h). However, cor15a-ipt chrysanthemum plants and excised leaves that were not exposed to a cold-induction period, senesced under the same dark storage conditions. Regardless of cold-induction treatment, leaves and plants of non-transformed plants senesced under prolonged dark storage. Analysis of ipt expression indicated a marked increase in gene expression in intact transgenic plants as well ... (en)
- Rostliny Petunia x hybrida ‘Marco Polo Odyssey’ a Dendranthema x grandiflorum (chrysanthemum) ‘Iridon’ byly transformovány genem kódujícím isopentenyltransferázu (ipt), hlavní enzym biosyntézy cytokininů, pod kontrolou chladem indukovatelného promotoru cor15a (z Arabidopsis thaliana). Cílem bylo zpomalit senescenci listů mladých transplantátů a řezaných květů během skladování ve tmě a chladu. Transgenní rostliny petunií a chryzantém obsahující cor15a-ipt (stejně jako listy izolované z těchto rostlin) inkubované krátce v chladu (72 h při 4 °C) zůstaly během delšího skladovacího období ve tmě (4 týdny při 25 °C) zelené a zdravé. Oproti tomu transgenní cor15a-ipt chryzantémy (rostliny i izolované listy), které nebyly vystaveny indukční chladové periodě, vykázaly za stejných skladovacích podmínek výrazné známky stárnutí, srovnatelné se senescencí kontrolních, netransformovaných rostlin (ať již s krátkou inkubací při 4 °C nebo bez ní). Analýza exprese ipt ukázala v intaktních trans... (cs)
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Title
| - Effects of cor15a-IPT gene expression on leaf senescence in transgenic Petuniaxhybrida and Dendranthemaxgrandiflorum
- Effects of cor15a-IPT gene expression on leaf senescence in transgenic Petuniaxhybrida and Dendranthemaxgrandiflorum (en)
- Vliv exprese genu cor15a-IPT na senescenci listů v transgenních rostlinách Petunia x hybrida a Dendranthema x grandiflorum (cs)
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skos:prefLabel
| - Effects of cor15a-IPT gene expression on leaf senescence in transgenic Petuniaxhybrida and Dendranthemaxgrandiflorum
- Effects of cor15a-IPT gene expression on leaf senescence in transgenic Petuniaxhybrida and Dendranthemaxgrandiflorum (en)
- Vliv exprese genu cor15a-IPT na senescenci listů v transgenních rostlinách Petunia x hybrida a Dendranthema x grandiflorum (cs)
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skos:notation
| - RIV/61389030:_____/05:00030612!RIV06-AV0-61389030
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(LN00A081), Z(AV0Z50380511)
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61389030:_____/05:00030612
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Cold-inducible promoter; cytokinins; ipt expression (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
| - Journal of Experimental Botany
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Li, J.
- Malbeck, Jiří
- Vaňková, Radomíra
- Li, Y.
- Khodakovskaya, M.
- McAvoy, R.
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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