About: Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: host´s RNA degradation

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About: Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: host´s RNA degradation Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Rostliny tabáku infikovaného Y-virem bramboru (PVY) byly studovány během akutní fáze infekce. Regulační enzymy degradace hostitelské RNA potřebné pro biosyntézu PVY-RNA, jejich hrubá a jemná regulace, a obsah hostitelské RNA byly monitorovány. Aktivity ribonukleáz, fosfomonoesteráz a fosfodiesteráz byly silně zvýšeny jak v hrubém homogenátu tak v částečném purifikátu daných enzymů z infikovaných listů ve srovnání s listy zdravých rostlin. Průběh enzymových aktivit negativně koreloval s obsahem hostitelské RNA. Aktivity enzymů v homogenátu se statisticky významně nelišily od aktivit v částečném purifikátu. (cs) Tobacco plants infected with the potato virus Y (PVY) were studied during the acute-infection period. The kontrol enzymes of metabolic pathway of host´s RNA degradation tending to biosynthesis of PVY-RNA, its coarse/fine regulation and content of host´s RNA were monitored. Activities of ribonucleases, phosphomonoesterases and phosphodiesterases in both the crude homogenates and the partially purified enzyme preparations from the diseased leaves were markedly increased when compared to the tissues from healthy plants. The curves of enzyme activities positively correlated with the multiplication curve of the PVY and negatively correlated with the decreased contents of host´s RNA. The enzyme activity in homogenate samples did not significantly differ from the corresponding purified enzyme preparations. Tobacco plants infected with the potato virus Y (PVY) were studied during the acute-infection period. The kontrol enzymes of metabolic pathway of host´s RNA degradation tending to biosynthesis of PVY-RNA, its coarse/fine regulation and content of host´s RNA were monitored. Activities of ribonucleases, phosphomonoesterases and phosphodiesterases in both the crude homogenates and the partially purified enzyme preparations from the diseased leaves were markedly increased when compared to the tissues from healthy plants. The curves of enzyme activities positively correlated with the multiplication curve of the PVY and negatively correlated with the decreased contents of host´s RNA. The enzyme activity in homogenate samples did not significantly differ from the corresponding purified enzyme preparations. (en)
Title	Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: host´s RNA degradation Regulace metabolických cest biosyntézy PVY-RNA v tabáku: degradace hostitelské RNA (cs) Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: host´s RNA degradation (en)
skos:prefLabel	Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: host´s RNA degradation Regulace metabolických cest biosyntézy PVY-RNA v tabáku: degradace hostitelské RNA (cs) Regulation of metabolic pathways PVY-RNA biosynthesis in tobacco: host´s RNA degradation (en)
skos:notation	RIV/61389030:_____/05:00028424!RIV06-GA0-61389030
http://linked.open.../vavai/riv/strany	309;312
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/02/0708), P(KSK5020115), Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2006
http://linked.open...aciTvurceVysledku	Šindelář, Luděk Šindelářová, Milada
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Ústav experimentální botaniky AV ČR, v. v. i.
http://linked.open...dnocenehoVysledku	540551
http://linked.open...ai/riv/idVysledku	RIV/61389030:_____/05:00028424
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Nicotiana tabacum L.; phosphodiesterase; phosphomonoesterase (en)
http://linked.open.../riv/klicoveSlovo	Nicotiana tabacum L. phosphodiesterase phosphomonoesterase
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[45C5DA2AC261]
http://linked.open...i/riv/nazevZdroje	Biologia Plantarum
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	2 (xsd:int)
http://linked.open...vavai/riv/projekt	Molecular basis of biological transformations, communications and technologies The role of chloroplasts in mechanisms involved in replication and translation of viral-RNA in connection with host resistance
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	49
http://linked.open...iv/tvurceVysledku	Šindelářová, Milada Šindelář, Luděk
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy
issn	0006-3134
number of pages	4 (xsd:int)

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